論文 - 詳細
| RRC ID | 41583 |
|---|---|
| 著者 | Nagashima K, Torii S, Yi Z, Igarashi M, Okamoto K, Takeuchi T, Izumi T. |
| タイトル | Melanophilin directly links Rab27a and myosin Va through its distinct coiled-coil regions. |
| ジャーナル | FEBS Lett |
| Abstract |
Rab GTPases regulate the membrane transport pathways by recruiting their specific effector proteins. Melanophilin, a putative Rab effector, has recently been identified as a gene that is mutated in leaden mice, in which peripheral localization of melanosomes is impaired in melanocytes. Genetic studies suggest that three coat-color mutation genes, dilute (MyoVa(d)), ashen (Rab27a(ash)), and leaden (Mlph(ln)), act in the same or overlapping pathways. Here we have cloned and characterized a human melanophilin homolog, which belongs to the rabphilin3/granuphilin-like Rab effector family. Cosedimentation assays using recombinant proteins reveal that melanophilin directly binds to Rab27a and myosin Va through its N-terminal and its first C-terminal coiled-coil region, respectively. Moreover, we show that Rab27a, melanophilin, and myosin Va form a ternary complex in the human melanocyte cell line HMV-II. These findings suggest that melanophilin has a role in bridging Rab27a on melanosomes and myosin Va on actin filaments during melanosome transport. We also propose that the Rab-binding region conserved in a novel rabphilin3/granuphilin-like Rab effector family constitutes an alpha-helix-based coiled-coil structure. |
| 巻・号 | 517(1-3) |
| ページ | 233-8 |
| 公開日 | 2002-4-24 |
| DOI | 10.1016/s0014-5793(02)02634-0 |
| PII | S0014579302026340 |
| PMID | 12062444 |
| MeSH | Actin Cytoskeleton / metabolism Adaptor Proteins, Signal Transducing Binding Sites Biological Transport / physiology Carrier Proteins / metabolism* Humans Macromolecular Substances Melanocytes / metabolism* Melanoma Melanosomes / metabolism Molecular Sequence Data Myosin Type V / chemistry Myosin Type V / metabolism* Protein Conformation Sequence Alignment Tumor Cells, Cultured rab GTP-Binding Proteins / chemistry rab GTP-Binding Proteins / metabolism* rab27 GTP-Binding Proteins |
| IF | 3.057 |
| 引用数 | 102 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HMV-II(RCB0777) |