論文 - 詳細
| RRC ID | 41686 |
|---|---|
| 著者 | Nakagawa Y, Okada S, Hatano M, Ebara M, Saisho H, Tokuhisa T. |
| タイトル | Downregulation of bcl-xL is relevant to UV-induced apoptosis in fibroblasts. |
| ジャーナル | J Biochem Mol Biol |
| Abstract |
Exposure to ultraviolet light (UV) induces apoptosis in mammalian cells. The caspase group of proteases is required for the apoptosis. This pathway is initiated by a release of cytochrome c from the mitochondria into the cytosol. Several Bcl-2 family proteins can regulate the release of cytochrome c by stabilizing the mitochondrial membrane. Here we show that expression of the endogenous bcl-xL was strongly downregulated in NIH3T3 cells within 2 h after UV-C irradiation, and that of bax was upregulated from 8 h after irradiation. Apoptosis was induced in more than 50% of the NIH3T3 cells 48 h after irradiation. Constitutive overexpression of bcl-xL in NIH3T3 cells protected the UV-induced apoptosis by preventing the loss of mitochondrial membrane potential and the activation of caspase 9. These results suggest that downregulation of Bcl-xL is relevant to UV-induced apoptosis of fibroblasts. |
| 巻・号 | 35(5) |
| ページ | 452-8 |
| 公開日 | 2002-9-30 |
| DOI | 10.5483/bmbrep.2002.35.5.452 |
| PMID | 12359085 |
| MeSH | 3T3 Cells Animals Annexin A5 / metabolism Apoptosis / radiation effects* Caspase 9 Caspases / metabolism Caspases / radiation effects Cell Survival / radiation effects Down-Regulation / radiation effects* Enzyme Activation Fibroblasts Membrane Potentials / radiation effects Mice Mitochondria / radiation effects Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins c-bcl-2 / genetics* Proto-Oncogene Proteins c-bcl-2 / physiology Proto-Oncogene Proteins c-bcl-2 / radiation effects Ultraviolet Rays* bcl-2-Associated X Protein bcl-X Protein |
| 引用数 | 12 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3 |