Reference - Detail
| RRC ID | 41705 |
|---|---|
| Author | Kuboyama T, Tohda C, Zhao J, Nakamura N, Hattori M, Komatsu K. |
| Title | Axon- or dendrite-predominant outgrowth induced by constituents from Ashwagandha. |
| Journal | Neuroreport |
| Abstract |
We previously reported that the methanol extract of Ashwagandha (roots of Dunal) induced dendrite extension in a human neuroblastoma cell line. In this study, we found that six of the 18 compounds isolated from the methanol extract enhanced neurite outgrowth in human neuroblastoma SH-SY5Y cells. Double immunostaining was performed in rat cortical neurons using antibodies to phosphorylated NF-H as an axonal marker, and to MAP2 as a dendritic marker. In withanolide A-treated cells, the length of NF-H-positive processes was significantly increased compared with vehicle-treated cells, whereas, the length of MAP2-positive processes was increased by withanosides IV and VI. These results suggest that axons are predominantly extended by withanolide A, and dendrites by withanosides IV and VI. |
| Volume | 13(14) |
| Pages | 1715-20 |
| Published | 2002-10-7 |
| DOI | 10.1097/00001756-200210070-00005 |
| PMID | 12395110 |
| MeSH | Animals Axons / drug effects* Axons / ultrastructure Cell Differentiation / drug effects* Cell Differentiation / physiology Cells, Cultured Cerebral Cortex / cytology Cerebral Cortex / drug effects* Cerebral Cortex / embryology* Dendrites / drug effects* Dendrites / ultrastructure Female Fetus Growth Substances / chemistry Growth Substances / pharmacology* Humans Immunohistochemistry Microtubule-Associated Proteins / drug effects Microtubule-Associated Proteins / metabolism Molecular Structure Neurites / drug effects Neurites / ultrastructure Neurofilament Proteins / drug effects Neurofilament Proteins / metabolism Plant Extracts / chemistry Plant Extracts / pharmacology* Pregnancy Rats Rats, Sprague-Dawley Tumor Cells, Cultured Withania / chemistry* |
| IF | 1.394 |
| Times Cited | 71 |
| WOS Category | NEUROSCIENCES |
| Resource | |
| Human and Animal Cells | |