RRC ID 4171
Author Eimer S, Gottschalk A, Hengartner M, Horvitz HR, Richmond J, Schafer WR, Bessereau JL.
Title Regulation of nicotinic receptor trafficking by the transmembrane Golgi protein UNC-50.
Journal EMBO J.
Abstract Nicotinic acetylcholine receptors (AChRs) are pentameric ligand-gated ion channels that mediate fast synaptic transmission at the neuromuscular junction (NMJ). After assembly in the endoplasmic reticulum (ER), AChRs must be transported to the plasma membrane through the secretory apparatus. Little is known about specific molecules that mediate this transport. Here we identify a gene that is required for subtype-specific trafficking of assembled nicotinic AChRs in Caenorhabditis elegans. unc-50 encodes an evolutionarily conserved integral membrane protein that localizes to the Golgi apparatus. In the absence of UNC-50, a subset of AChRs present in body-wall muscle are sorted to the lysosomal system and degraded. However, the trafficking of a second AChR type and of GABA ionotropic receptors expressed in the same muscle cells is not affected in unc-50 mutants. These results suggest that, in addition to ER quality control, assembled AChRs are sorted within the Golgi system by a mechanism that controls the amount of cell-surface AChRs in a subtype-specific way.
Volume 26(20)
Pages 4313-23
Published 2007-10-17
DOI 10.1038/sj.emboj.7601858
PII 7601858
PMID 17853888
PMC PMC2034668
MeSH Amino Acid Sequence Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology Cell Membrane / metabolism Endoplasmic Reticulum / metabolism Golgi Apparatus / metabolism* Lysosomes / metabolism Membrane Proteins / chemistry Membrane Proteins / physiology* Models, Biological Molecular Sequence Data Mutation Protein Conformation Receptors, Nicotinic / metabolism* Sequence Homology, Amino Acid
IF 11.227
Times Cited 38
C.elegans ?