| RRC ID |
41712
|
| 著者 |
Zhang Y, Iwasaki H, Wang H, Kudo T, Kalka TB, Hennet T, Kubota T, Cheng L, Inaba N, Gotoh M, Togayachi A, Guo J, Hisatomi H, Nakajima K, Nishihara S, Nakamura M, Marth JD, Narimatsu H.
|
| タイトル |
Cloning and characterization of a new human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, designated pp-GalNAc-T13, that is specifically expressed in neurons and synthesizes GalNAc alpha-serine/threonine antigen.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
To date, 10 members of the UDP-N-acetyl-alpha-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) family have been cloned and analyzed in human. In this study, we cloned and analyzed a novel human pp-GalNAc-T from an NT2 cell cDNA library, and we named it pp-GalNAc-T13. In amino acid sequences, pp-GalNAc-T13 was highly homologous, showing 84.3% identity, to pp-GalNAc-T1. Real time PCR analysis revealed pp-GalNAc-T13 to be highly and restrictively expressed in the brain and present at very low or undetectable levels in other tissues, in contrast to the ubiquitous expression of pp-GalNAc-T1. pp-GalNAc-T13 was abundantly expressed in all neuroblastoma cells examined and primary cultured neurons but not in glioblastoma cells and primary cultured astrocytes. pp-GalNAc-T13 exhibited much stronger activity to transfer GalNAc to mucin peptides, such as Muc5Ac and MUC7, than did pp-GalNAc-T1. In addition, pp-GalNAc-T13 differed in substrate specificity to pp-GalNAc-T1. pp-GalNAc-T13 was able to form a triplet Tn epitope, three consecutive GalNAc-Ser/Thr structures, on peptides encoded in syndecan-3, a proteoglycan expressed in neurons. pp-GalNAc-T13-deficient mice have been established in a previous work. Immunohistochemical study showed a remarkable decrease in Tn antigen expression in the cerebellum of the pp-GalNAc-T13 knockout mouse. pp-GalNAc-T13 would be a major enzyme responsible for the synthesis of O-glycan and specifically the Tn antigen epitope in neurons.
|
| 巻・号 |
278(1)
|
| ページ |
573-84
|
| 公開日 |
2003-1-3
|
| DOI |
10.1074/jbc.M203094200
|
| PII |
S0021-9258(19)31307-9
|
| PMID |
12407114
|
| MeSH |
Amino Acid Sequence
Animals
Antigens, Tumor-Associated, Carbohydrate / immunology
Antigens, Tumor-Associated, Carbohydrate / metabolism*
Astrocytes / cytology
Astrocytes / metabolism
Base Sequence
Cells, Cultured
Cerebellar Cortex / cytology
Cerebellar Cortex / metabolism
Chromatography, High Pressure Liquid
Cloning, Molecular
Glycopeptides / metabolism
Glycosylation
Humans
Membrane Glycoproteins / metabolism
Mice
Mice, Knockout
Molecular Sequence Data
Mucin 5AC
Mucins / metabolism
N-Acetylgalactosaminyltransferases / genetics
N-Acetylgalactosaminyltransferases / metabolism*
Neurons / cytology
Neurons / physiology*
Polypeptide N-acetylgalactosaminyltransferase
Proteoglycans / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sequence Alignment
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substrate Specificity
Syndecan-3
Tissue Distribution
|
| IF |
4.238
|
| 引用数 |
96
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
KG-1-C(RCB02700 |
