| RRC ID |
4171
|
| Author |
Eimer S, Gottschalk A, Hengartner M, Horvitz HR, Richmond J, Schafer WR, Bessereau JL.
|
| Title |
Regulation of nicotinic receptor trafficking by the transmembrane Golgi protein UNC-50.
|
| Journal |
EMBO J
|
| Abstract |
Nicotinic acetylcholine receptors (AChRs) are pentameric ligand-gated ion channels that mediate fast synaptic transmission at the neuromuscular junction (NMJ). After assembly in the endoplasmic reticulum (ER), AChRs must be transported to the plasma membrane through the secretory apparatus. Little is known about specific molecules that mediate this transport. Here we identify a gene that is required for subtype-specific trafficking of assembled nicotinic AChRs in Caenorhabditis elegans. unc-50 encodes an evolutionarily conserved integral membrane protein that localizes to the Golgi apparatus. In the absence of UNC-50, a subset of AChRs present in body-wall muscle are sorted to the lysosomal system and degraded. However, the trafficking of a second AChR type and of GABA ionotropic receptors expressed in the same muscle cells is not affected in unc-50 mutants. These results suggest that, in addition to ER quality control, assembled AChRs are sorted within the Golgi system by a mechanism that controls the amount of cell-surface AChRs in a subtype-specific way.
|
| Volume |
26(20)
|
| Pages |
4313-23
|
| Published |
2007-10-17
|
| DOI |
10.1038/sj.emboj.7601858
|
| PII |
7601858
|
| PMID |
17853888
|
| PMC |
PMC2034668
|
| MeSH |
Amino Acid Sequence
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / metabolism*
Caenorhabditis elegans Proteins / physiology
Cell Membrane / metabolism
Endoplasmic Reticulum / metabolism
Golgi Apparatus / metabolism*
Lysosomes / metabolism
Membrane Proteins / chemistry
Membrane Proteins / physiology*
Models, Biological
Molecular Sequence Data
Mutation
Protein Conformation
Receptors, Nicotinic / metabolism*
Sequence Homology, Amino Acid
|
| IF |
9.889
|
| Times Cited |
48
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
|
