RRC ID |
4172
|
著者 |
Frische EW, Pellis-van Berkel W, van Haaften G, Cuppen E, Plasterk RH, Tijsterman M, Bos JL, Zwartkruis FJ.
|
タイトル |
RAP-1 and the RAL-1/exocyst pathway coordinate hypodermal cell organization in Caenorhabditis elegans.
|
ジャーナル |
EMBO J
|
Abstract |
The small Ras-like GTPase Rap1 has been identified as a regulator of integrin activation and cadherin-mediated cell-cell contacts. Surprisingly, null mutants of RAP-1 in Caenorhabditis elegans are viable and fertile. In a synthetic lethal RNAi screen with C. elegans rap-1 mutants, the Ras-like GTPase ral-1 emerged as one of seven genes specifically required for viability. Depletion of exoc-8 and sec-5, encoding two putative RAL-1 effectors and members of the exocyst complex, also caused lethality of rap-1 mutants, but did not affect wild-type worms. The RAP-1 and the RAL-1/exocyst pathway appear to coordinate hypodermal cell movement and elongation during embryonic development. They mediate their effect in part through targeting the alpha-catenin homologue HMP-1 to the lateral membrane. Genetic interactions show that the RAP-1 and RAL-1/exocyst pathway also act in parallel during larval stages. Together these data provide in vivo evidence for the exocyst complex as a downstream RAL-1 effector in cell migration.
|
巻・号 |
26(24)
|
ページ |
5083-92
|
公開日 |
2007-12-12
|
DOI |
10.1038/sj.emboj.7601922
|
PII |
7601922
|
PMID |
17989692
|
PMC |
PMC2140109
|
MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / cytology
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Movement / physiology*
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / metabolism
Embryo, Nonmammalian / anatomy & histology
Embryo, Nonmammalian / physiology
Mutation
Phenotype
RNA Interference
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Subcutaneous Tissue*
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*
ral GTP-Binding Proteins / genetics
ral GTP-Binding Proteins / metabolism*
rap1 GTP-Binding Proteins / genetics
rap1 GTP-Binding Proteins / metabolism*
|
IF |
9.889
|
引用数 |
27
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
線虫 |
tm861
tm1443 |