RRC ID 4172
Author Frische EW, Pellis-van Berkel W, van Haaften G, Cuppen E, Plasterk RH, Tijsterman M, Bos JL, Zwartkruis FJ.
Title RAP-1 and the RAL-1/exocyst pathway coordinate hypodermal cell organization in Caenorhabditis elegans.
Journal EMBO J.
Abstract The small Ras-like GTPase Rap1 has been identified as a regulator of integrin activation and cadherin-mediated cell-cell contacts. Surprisingly, null mutants of RAP-1 in Caenorhabditis elegans are viable and fertile. In a synthetic lethal RNAi screen with C. elegans rap-1 mutants, the Ras-like GTPase ral-1 emerged as one of seven genes specifically required for viability. Depletion of exoc-8 and sec-5, encoding two putative RAL-1 effectors and members of the exocyst complex, also caused lethality of rap-1 mutants, but did not affect wild-type worms. The RAP-1 and the RAL-1/exocyst pathway appear to coordinate hypodermal cell movement and elongation during embryonic development. They mediate their effect in part through targeting the alpha-catenin homologue HMP-1 to the lateral membrane. Genetic interactions show that the RAP-1 and RAL-1/exocyst pathway also act in parallel during larval stages. Together these data provide in vivo evidence for the exocyst complex as a downstream RAL-1 effector in cell migration.
Volume 26(24)
Pages 5083-92
Published 2007-12-12
DOI 10.1038/sj.emboj.7601922
PII 7601922
PMID 17989692
PMC PMC2140109
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Movement / physiology* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism Embryo, Nonmammalian / anatomy & histology Embryo, Nonmammalian / physiology Mutation Phenotype RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Subcutaneous Tissue* Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* ral GTP-Binding Proteins / genetics ral GTP-Binding Proteins / metabolism* rap1 GTP-Binding Proteins / genetics rap1 GTP-Binding Proteins / metabolism*
IF 10.557
Times Cited 24
C.elegans tm861 tm1443