RRC ID 41741
著者 Lee SW, Kwak HB, Lee HC, Lee SK, Kim HH, Lee ZH.
タイトル The anti-proliferative gene TIS21 is involved in osteoclast differentiation.
ジャーナル J Biochem Mol Biol
Abstract The remodeling process of bone is accompanied by complex changes in the expression levels of various genes. Several approaches have been employed to detect differentially-expressed genes in regard to osteoclast differentiation. In order to identify the genes that are involved in osteoclast differentiation, we used a cDNAarray-nylon membrane. Among 1,200 genes that showed a measurable signal, 19 genes were chosen for further study. Eleven genes were up-regulated; eight genes were downregulated. TIS21 was one of the up-regulated genes which were highly expressed in mature osteoclasts. To verify the cDNA microarray results, we carried out RT-PCR and real-time RT-PCR for the TIS21 gene. The TIS21 mRNA level was higher in differentiated-osteoclasts when compared to undifferentiated bone-marrow macrophages. Furthermore, the treatment with 1 mM of a TIS21 antisense oligonucleotide reduced the formation of osteoclasts from the bone-marrow-precursor cells by approximately 30%. These results provide evidence for the potential role of TIS21 in the differentiation of osteoclasts
巻・号 35(6)
ページ 609-14
公開日 2002-11-30
DOI 10.5483/bmbrep.2002.35.6.609
PMID 12470596
MeSH Animals Cell Differentiation Cell Line Cells, Cultured DNA, Complementary / metabolism Down-Regulation Genes, Tumor Suppressor* Immediate-Early Proteins / chemistry* Immediate-Early Proteins / genetics Immediate-Early Proteins / physiology* Macrophages / metabolism Mice Oligonucleotide Array Sequence Analysis Oligonucleotides, Antisense / pharmacology Osteoclasts / cytology* Reverse Transcriptase Polymerase Chain Reaction Tumor Suppressor Proteins Up-Regulation
引用数 6
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)