RRC ID |
41741
|
著者 |
Lee SW, Kwak HB, Lee HC, Lee SK, Kim HH, Lee ZH.
|
タイトル |
The anti-proliferative gene TIS21 is involved in osteoclast differentiation.
|
ジャーナル |
J Biochem Mol Biol
|
Abstract |
The remodeling process of bone is accompanied by complex changes in the expression levels of various genes. Several approaches have been employed to detect differentially-expressed genes in regard to osteoclast differentiation. In order to identify the genes that are involved in osteoclast differentiation, we used a cDNAarray-nylon membrane. Among 1,200 genes that showed a measurable signal, 19 genes were chosen for further study. Eleven genes were up-regulated; eight genes were downregulated. TIS21 was one of the up-regulated genes which were highly expressed in mature osteoclasts. To verify the cDNA microarray results, we carried out RT-PCR and real-time RT-PCR for the TIS21 gene. The TIS21 mRNA level was higher in differentiated-osteoclasts when compared to undifferentiated bone-marrow macrophages. Furthermore, the treatment with 1 mM of a TIS21 antisense oligonucleotide reduced the formation of osteoclasts from the bone-marrow-precursor cells by approximately 30%. These results provide evidence for the potential role of TIS21 in the differentiation of osteoclasts
|
巻・号 |
35(6)
|
ページ |
609-14
|
公開日 |
2002-11-30
|
DOI |
10.5483/bmbrep.2002.35.6.609
|
PMID |
12470596
|
MeSH |
Animals
Cell Differentiation
Cell Line
Cells, Cultured
DNA, Complementary / metabolism
Down-Regulation
Genes, Tumor Suppressor*
Immediate-Early Proteins / chemistry*
Immediate-Early Proteins / genetics
Immediate-Early Proteins / physiology*
Macrophages / metabolism
Mice
Oligonucleotide Array Sequence Analysis
Oligonucleotides, Antisense / pharmacology
Osteoclasts / cytology*
Reverse Transcriptase Polymerase Chain Reaction
Tumor Suppressor Proteins
Up-Regulation
|
引用数 |
6
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
RAW 264(RCB0535) |