論文 - 詳細
| RRC ID | 41741 |
|---|---|
| 著者 | Lee SW, Kwak HB, Lee HC, Lee SK, Kim HH, Lee ZH. |
| タイトル | The anti-proliferative gene TIS21 is involved in osteoclast differentiation. |
| ジャーナル | J Biochem Mol Biol |
| Abstract |
The remodeling process of bone is accompanied by complex changes in the expression levels of various genes. Several approaches have been employed to detect differentially-expressed genes in regard to osteoclast differentiation. In order to identify the genes that are involved in osteoclast differentiation, we used a cDNAarray-nylon membrane. Among 1,200 genes that showed a measurable signal, 19 genes were chosen for further study. Eleven genes were up-regulated; eight genes were downregulated. TIS21 was one of the up-regulated genes which were highly expressed in mature osteoclasts. To verify the cDNA microarray results, we carried out RT-PCR and real-time RT-PCR for the TIS21 gene. The TIS21 mRNA level was higher in differentiated-osteoclasts when compared to undifferentiated bone-marrow macrophages. Furthermore, the treatment with 1 mM of a TIS21 antisense oligonucleotide reduced the formation of osteoclasts from the bone-marrow-precursor cells by approximately 30%. These results provide evidence for the potential role of TIS21 in the differentiation of osteoclasts |
| 巻・号 | 35(6) |
| ページ | 609-14 |
| 公開日 | 2002-11-30 |
| DOI | 10.5483/bmbrep.2002.35.6.609 |
| PMID | 12470596 |
| MeSH | Animals Cell Differentiation Cell Line Cells, Cultured DNA, Complementary / metabolism Down-Regulation Genes, Tumor Suppressor* Immediate-Early Proteins / chemistry* Immediate-Early Proteins / genetics Immediate-Early Proteins / physiology* Macrophages / metabolism Mice Oligonucleotide Array Sequence Analysis Oligonucleotides, Antisense / pharmacology Osteoclasts / cytology* Reverse Transcriptase Polymerase Chain Reaction Tumor Suppressor Proteins Up-Regulation |
| 引用数 | 6 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |