論文 - 詳細
| RRC ID | 41749 |
|---|---|
| 著者 | Araki N, Hatae T, Furukawa A, Swanson JA. |
| タイトル | Phosphoinositide-3-kinase-independent contractile activities associated with Fcgamma-receptor-mediated phagocytosis and macropinocytosis in macrophages. |
| ジャーナル | J Cell Sci |
| Abstract |
Previous studies have shown that Fcgamma receptor (FcR)-mediated phagocytosis and macropinocytosis in macrophages consist of two dissociable activities: a phosphoinositide 3-kinase (PI3K)-independent extension of phagocytic cups and a PI3K-dependent contractile mechanism that closes phagosomes and ruffles into intracellular organelles. Here, we identify an additional contractile activity that persists in the presence of the PI3K inhibitor wortmannin. ML-7, an inhibitor of myosin-light-chain kinase (MLCK), inhibited FcR-mediated phagocytosis, macropinocytosis and cell movements associated with ruffling. Scanning electron microscopy demonstrated a striking difference in morphology between phagocytic cups in the different inhibitors: whereas phagocytic cups of control cells and wortmannin-treated cells conformed closely to particles and appeared to have constricted them, the phagocytic cups in cells treated with ML-7 were more open. Video microscopy of macrophages expressing green-fluorescent-protein (GFP)-actin fusions revealed that bound IgG-opsonized erythrocytes were squeezed during phagosome formation and closure. In ML-7, GFP-actin-rich protrusions extended outward but failed to squeeze particles. Moreover, in contrast to the effects of PI3K inhibitors, ML-7 markedly reduced ruffle movement, and perturbed circular ruffle formation. These PI3K-independent myosin-II-based contractile activities that squeeze phagocytic cups and curve ruffles therefore represent a third component activity of the actin cytoskeleton during phagocytosis and macropinocytosis. |
| 巻・号 | 116(Pt 2) |
| ページ | 247-57 |
| 公開日 | 2003-1-15 |
| DOI | 10.1242/jcs.00235 |
| PMID | 12482911 |
| MeSH | Actin Cytoskeleton / drug effects Actin Cytoskeleton / genetics Actin Cytoskeleton / metabolism Actin Cytoskeleton / ultrastructure Androstadienes / pharmacology Animals Azepines / pharmacology Cell Surface Extensions / drug effects Cell Surface Extensions / metabolism Cell Surface Extensions / ultrastructure Cells, Cultured Contractile Proteins / drug effects Contractile Proteins / metabolism* Enzyme Inhibitors / pharmacology Erythrocytes / cytology Erythrocytes / metabolism Macrophages / drug effects Macrophages / enzymology* Macrophages / ultrastructure Mice Microscopy, Electron, Scanning Myosin-Light-Chain Kinase / antagonists & inhibitors Myosin-Light-Chain Kinase / metabolism Myosins / metabolism Naphthalenes / pharmacology Phagocytosis / drug effects Phagocytosis / physiology* Phosphatidylinositol 3-Kinases / metabolism* Phosphoinositide-3 Kinase Inhibitors Pinocytosis / drug effects Pinocytosis / physiology* Receptors, IgG / drug effects Receptors, IgG / metabolism* Recombinant Fusion Proteins Wortmannin |
| IF | 4.573 |
| 引用数 | 137 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |