| RRC ID |
41753
|
| 著者 |
Yamashita Y, Shimada M, Harimoto N, Rikimaru T, Shirabe K, Tanaka S, Sugimachi K.
|
| タイトル |
Histone deacetylase inhibitor trichostatin A induces cell-cycle arrest/apoptosis and hepatocyte differentiation in human hepatoma cells.
|
| ジャーナル |
Int J Cancer
|
| Abstract |
Remodeling of the chromatin template by inhibition of HDAC activities represents a potential transcriptional therapy for neoplastic disease. A number of HDAC inhibitors that modulate in vitro cell growth and differentiation have been developed. We analyzed the effects of TSA, a specific and potent HDAC inhibitor, on the human hepatoma cell lines HepG2 and Huh-7. TSA increased levels of acetylated histones H3 and H4 in both HepG2 and Huh-7. It inhibited cell proliferation in vitro and induced G(0)/G(1) arrest in HepG2 and apoptosis in Huh-7. Gene expression of liver-specific functions and liver-enriched transcription factors was upregulated by TSA. TSA upregulated the ammonia removal rate and the albumin synthesis rate of HepG2 and Huh-7. Our results indicate that TSA can induce cell-cycle arrest/apoptosis and hepatocyte differentiation in human liver cancer cell lines.
|
| 巻・号 |
103(5)
|
| ページ |
572-6
|
| 公開日 |
2003-2-20
|
| DOI |
10.1002/ijc.10699
|
| PMID |
12494463
|
| MeSH |
Albumins / metabolism
Ammonia / metabolism
Apoptosis / drug effects*
Carcinoma, Hepatocellular / enzymology
Carcinoma, Hepatocellular / pathology*
Cell Cycle / drug effects
Cell Differentiation / drug effects
Cell Division / drug effects
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology*
Gene Expression Regulation, Neoplastic
Hepatocytes / drug effects*
Hepatocytes / metabolism
Histone Deacetylase Inhibitors*
Histones / metabolism
Humans
Hydroxamic Acids / pharmacology*
Liver Neoplasms / enzymology
Liver Neoplasms / pathology*
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
Tumor Cells, Cultured / drug effects
Tumor Cells, Cultured / enzymology
Tumor Cells, Cultured / pathology
|
| IF |
5.145
|
| 引用数 |
132
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2
HuH-7 |
