論文 - 詳細
| RRC ID | 41761 |
|---|---|
| 著者 | Omori S, Takiguchi Y, Suda A, Sugimoto T, Miyazawa H, Takiguchi Y, Tanabe N, Tatsumi K, Kimura H, Pardington PE, Chen F, Chen DJ, Kuriyama T. |
| タイトル | Suppression of a DNA double-strand break repair gene, Ku70, increases radio- and chemosensitivity in a human lung carcinoma cell line. |
| ジャーナル | DNA Repair (Amst) |
| Abstract |
Ku70 protein, cooperating with Ku80 and DNA-dependent protein kinase (DNA-PK) catalytic subunit (DNA-PKcs), is involved in DNA double-strand break (DNA DSB) repair and V(D)J recombination. Recent studies have revealed increased ionizing radiosensitivity in Ku70-deficient cells. The presented study, using a human squamous cell lung carcinoma cell line, demonstrated that introduction of an antisense Ku70 nucleic acid made the cells more radio- and chemosensitive than the parental cells. Ku70 protein expression was suppressed in the cells with antisense Ku70 construct when compared to the wild-type cells. A relatively small but statistically significant increase in radiosensitivity of the cells was achieved by the introduction of the antisense Ku70. The increased radiosensitivity in vitro was accompanied by an approximately two-fold increase in alpha and alpha/beta values in a linear-quadratic model. The antisense Ku70 increased the chemosensitivity of the cells to some DNA-damaging agents such as bleomycin and methyl methanesulfonate, but not to cisplatin, mitomycin C, and paclitaxel. This system provides us with partial suppression of Ku70, and will be a useful experimental model for investigating the physiological roles of the DNA DSB repair gene. |
| 巻・号 | 1(4) |
| ページ | 299-310 |
| 公開日 | 2002-4-29 |
| DOI | 10.1016/s1568-7864(02)00006-x |
| PII | S156878640200006X |
| PMID | 12509248 |
| MeSH | Antigens, Nuclear / genetics Antineoplastic Agents / therapeutic use Blotting, Northern Carcinoma, Squamous Cell / drug therapy* Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / radiotherapy* Colony-Forming Units Assay DNA Damage DNA Helicases* DNA Primers DNA Repair / drug effects* DNA Repair / genetics DNA, Antisense / pharmacology DNA, Neoplasm / drug effects DNA-Binding Proteins / antagonists & inhibitors* DNA-Binding Proteins / genetics Electrophoretic Mobility Shift Assay Humans In Vitro Techniques Ku Autoantigen Lung Neoplasms / drug therapy* Lung Neoplasms / genetics Lung Neoplasms / radiotherapy* Transfection Tumor Cells, Cultured / drug effects Tumor Cells, Cultured / radiation effects X-Rays |
| IF | 3.339 |
| 引用数 | 63 |
| WOS 分野 | TOXICOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RERF-LC-AI(RCB0444) |