RRC ID 41835
Author Asano A, Jin HK, Watanabe T.
Title Mouse Mx2 gene: organization, mRNA expression and the role of the interferon-response promoter in its regulation.
Journal Gene
Abstract Mx is an interferon (IFN)-inducible intracellular protein found in various vertebrates that mediates resistance against negative-strand RNA viruses. We have demonstrated previously that feral mouse strains are able to express a functional Mx2 mRNA, while that of the inbred laboratory strains was non-functional because of a single nucleotide insertion in the open reading frame, and under the detectable level. In the present study, we examined the regulation of Mx2 expression in vivo using a congenic mouse carrying Mx1 and Mx2 genes derived from feral strain SPR. Mx2 mRNA was induced strongly in the spleen, ovary and white adipose tissue after the treatment with IFNalpha/beta. Furthermore, we identified the structure of the Mx2 gene. It consists of 14 exons, greatly homologous to the Mx1 gene. The promoter region of Mx2 contained two putative IFN-stimulated response elements (ISREs). We found that the proximal ISRE site positioned between -69 and -55 was essential to IFNalpha/beta-induced transcription by transient transfection assay using reporter gene constructs with mutants of the Mx2 promoter. These results indicate the similarity of the mechanisms of mRNA induction among Mx genes.
Volume 306
Pages 105-13
Published 2003-3-13
DOI 10.1016/s0378-1119(03)00428-1
PII S0378111903004281
PMID 12657472
MeSH 3T3 Cells 5' Flanking Region / genetics Animals Base Sequence Cells, Cultured Cloning, Molecular Cycloheximide / pharmacology DNA / chemistry DNA / genetics Dactinomycin / pharmacology Embryo, Mammalian / cytology Embryo, Mammalian / metabolism Exons Female Fibroblasts / cytology Fibroblasts / metabolism GTP-Binding Proteins / genetics* Gene Expression Gene Expression Regulation / drug effects Gene Expression Regulation, Developmental / drug effects Genes / genetics Interferons / pharmacology* Introns Luciferases / genetics Luciferases / metabolism Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred Strains Molecular Sequence Data Muridae Mutation Myxovirus Resistance Proteins Promoter Regions, Genetic / genetics* RNA, Messenger / genetics RNA, Messenger / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Response Elements / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid
IF 2.984
Times Cited 36
Human and Animal Cells