論文 - 詳細
| RRC ID | 41835 |
|---|---|
| 著者 | Asano A, Jin HK, Watanabe T. |
| タイトル | Mouse Mx2 gene: organization, mRNA expression and the role of the interferon-response promoter in its regulation. |
| ジャーナル | Gene |
| Abstract |
Mx is an interferon (IFN)-inducible intracellular protein found in various vertebrates that mediates resistance against negative-strand RNA viruses. We have demonstrated previously that feral mouse strains are able to express a functional Mx2 mRNA, while that of the inbred laboratory strains was non-functional because of a single nucleotide insertion in the open reading frame, and under the detectable level. In the present study, we examined the regulation of Mx2 expression in vivo using a congenic mouse carrying Mx1 and Mx2 genes derived from feral strain SPR. Mx2 mRNA was induced strongly in the spleen, ovary and white adipose tissue after the treatment with IFNalpha/beta. Furthermore, we identified the structure of the Mx2 gene. It consists of 14 exons, greatly homologous to the Mx1 gene. The promoter region of Mx2 contained two putative IFN-stimulated response elements (ISREs). We found that the proximal ISRE site positioned between -69 and -55 was essential to IFNalpha/beta-induced transcription by transient transfection assay using reporter gene constructs with mutants of the Mx2 promoter. These results indicate the similarity of the mechanisms of mRNA induction among Mx genes. |
| 巻・号 | 306 |
| ページ | 105-13 |
| 公開日 | 2003-3-13 |
| DOI | 10.1016/s0378-1119(03)00428-1 |
| PII | S0378111903004281 |
| PMID | 12657472 |
| MeSH | 3T3 Cells 5' Flanking Region / genetics Animals Base Sequence Cells, Cultured Cloning, Molecular Cycloheximide / pharmacology DNA / chemistry DNA / genetics Dactinomycin / pharmacology Embryo, Mammalian / cytology Embryo, Mammalian / metabolism Exons Female Fibroblasts / cytology Fibroblasts / metabolism GTP-Binding Proteins / genetics* Gene Expression Gene Expression Regulation / drug effects Gene Expression Regulation, Developmental / drug effects Genes / genetics Interferons / pharmacology* Introns Luciferases / genetics Luciferases / metabolism Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred Strains Molecular Sequence Data Muridae Mutation Myxovirus Resistance Proteins Promoter Regions, Genetic / genetics* RNA, Messenger / genetics RNA, Messenger / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Response Elements / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid |
| IF | 2.984 |
| 引用数 | 36 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |