| RRC ID |
41884
|
| Author |
Yamazaki K, Yamada E, Kanaji Y, Yanagisawa T, Kato Y, Sato K, Takano K, Sakasegawa Y, Kaneko K.
|
| Title |
Stimulation of cellular prion protein expression by TSH in human thyrocytes.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The cellular isoform of prion protein (PrP(C)) is a cell-surface glycosyl-phosphatidylinositol-anchored protein which is ubiquitously expressed on the cell membrane. It may function as a cell receptor or as a cell adhesion molecule. Thyroid follicles, obtained from patients with Graves' disease at thyroidectomy, were cultured in F-12/RPMI-1640 medium supplemented with 0.5% fetal bovine serum and bovine thyroid stimulating hormone (bTSH). Northern blot analyses revealed that bTSH increased the steady-state expression levels of PrP mRNA in a time- and dose-dependent manner. This increase was reproduced by dibutyryl-cAMP and 12-decanoylphorbol-13-acetate. The mRNA expression was greater in thyroid follicles in suspension culture than in thyrocytes cultured in a monolayer. These findings suggest that TSH stimulates PrP mRNA expression in thyrocytes through the protein kinase A and C pathways. The greater mRNA expression in thyroid follicles than in monolayer cells suggests that PrP(C) may be involved in structure formation or maintenance of thyroid follicles.
|
| Volume |
305(4)
|
| Pages |
1034-9
|
| Published |
2003-6-13
|
| DOI |
10.1016/s0006-291x(03)00801-5
|
| PII |
S0006291X03008015
|
| PMID |
12767934
|
| MeSH |
Animals
Cells, Cultured
Gene Expression Profiling
Gene Expression Regulation
Graves Disease / genetics
Graves Disease / metabolism
Humans
Mice
Oligonucleotide Array Sequence Analysis
PrPC Proteins / biosynthesis*
PrPC Proteins / genetics
RNA, Messenger / biosynthesis
Thyroid Gland / anatomy & histology
Thyroid Gland / drug effects
Thyroid Gland / metabolism*
Thyrotropin / pharmacology*
Tumor Cells, Cultured
Up-Regulation
|
| IF |
2.985
|
| Times Cited |
5
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
NB9(RCB0477)
NB69(RCB0480)
K562 |
