| RRC ID |
41894
|
| 著者 |
Koide N, Sugiyama T, Mori I, Mu MM, Yoshida T, Yokochi T.
|
| タイトル |
C2-ceramide inhibits LPS-induced nitric oxide production in RAW 264.7 macrophage cells through down-regulating the activation of Akt.
|
| ジャーナル |
J Endotoxin Res
|
| Abstract |
The effect of C(2)-ceramide, a membrane-permeable ceramide analogue, on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was studied. The non-toxic concentration of C(2)-ceramide inhibited LPS-induced NO production. It was due to the attenuated expression of the inducible type of NO synthase (iNOS). C(2)-ceramide did not influence the phosphorylation of a series of mitogen-activated protein (MAP) kinases in response to LPS. On the other hand, C(2)-ceramide down-regulated the phosphorylation of Akt in LPS-stimulated RAW 264.7 cells, followed by the impairment of nuclear factor (NF)-kappaB activation. Moreover, the Akt dominant-negative mutant inhibited LPS-induced NO production. C(2)-ceramide was suggested to inhibit LPS-induced NO production through down-regulating the activation of Akt.
|
| 巻・号 |
9(2)
|
| ページ |
85-90
|
| 公開日 |
2003-1-1
|
| DOI |
10.1179/096805103125001450
|
| PMID |
12803880
|
| MeSH |
Animals
Cell Line
Down-Regulation
Enzyme Inhibitors / pharmacology*
Escherichia coli / immunology
Lipopolysaccharides / pharmacology*
Macrophages / drug effects*
Macrophages / metabolism
Mice
Mitogen-Activated Protein Kinases / metabolism
NF-kappa B / biosynthesis
Nitric Oxide / metabolism*
Nitric Oxide Synthase / antagonists & inhibitors
Nitric Oxide Synthase Type II
Phosphorylation
Protein Serine-Threonine Kinases*
Proto-Oncogene Proteins / biosynthesis*
Proto-Oncogene Proteins c-akt
Sphingosine / analogs & derivatives*
Sphingosine / pharmacology*
Transfection
|
| 引用数 |
18
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
IMMUNOLOGY
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
