RRC ID 4195
著者 Zhang L, Ding L, Cheung TH, Dong MQ, Chen J, Sewell AK, Liu X, Yates JR 3rd, Han M.
タイトル Systematic identification of C. elegans miRISC proteins, miRNAs, and mRNA targets by their interactions with GW182 proteins AIN-1 and AIN-2.
ジャーナル Mol Cell
Abstract MicroRNAs (miRNAs) regulate gene expression for diverse functions, but only a limited number of mRNA targets have been experimentally identified. We show that GW182 family proteins AIN-1 and AIN-2 act redundantly to regulate the expression of miRNA targets, but not miRNA biogenesis. Immunoprecipitation (IP) and mass spectrometry indicate that AIN-1 and AIN-2 interact only with miRNA-specific Argonaute proteins ALG-1 and ALG-2 and with components of the core translational initiation complex. Known miRNA targets are enriched in AIN-2 complexes, correlating with the expression of corresponding miRNAs. Combining IP with pyrosequencing and microarray analysis of RNAs associated with AIN-1/AIN-2, we identified 106 previously annotated miRNAs plus nine new candidate miRNAs, but nearly no siRNAs, and more than 3500 potential miRNA targets, including nearly all known ones. Our results demonstrate an effective biochemical approach to systematically identify miRNA targets and provide valuable insights regarding the properties of miRNA effector complexes.
巻・号 28(4)
ページ 598-613
公開日 2007-11-30
DOI 10.1016/j.molcel.2007.09.014
PII S1097-2765(07)00626-0
PMID 18042455
PMC PMC2186060
MeSH 3' Untranslated Regions / metabolism Animals Base Sequence Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Endoribonucleases / metabolism Eukaryotic Initiation Factor-4E / metabolism Gene Expression Regulation, Developmental MicroRNAs / metabolism* Molecular Sequence Data Mutation / genetics Protein Binding Protein Biosynthesis RNA Caps / metabolism RNA, Helminth / isolation & purification RNA, Helminth / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / metabolism RNA-Induced Silencing Complex / metabolism* Sequence Analysis, RNA Time Factors
IF 15.584
引用数 181
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1863