論文 - 詳細
| RRC ID | 42036 |
|---|---|
| 著者 | Tu SP, Jiang XH, Lin MC, Cui JT, Yang Y, Lum CT, Zou B, Zhu YB, Jiang SH, Wong WM, Chan AO, Yuen MF, Lam SK, Kung HF, Wong BC. |
| タイトル | Suppression of survivin expression inhibits in vivo tumorigenicity and angiogenesis in gastric cancer. |
| ジャーナル | Cancer Res |
| Abstract |
Survivin plays an important role in cancer development. We aim to show here that suppression of survivin expression or function by antisense and dominant-negative (DN) mutant can inhibit gastric cancer carcinogenesis and angiogenesis in vivo. Plasmid constructs expressing survivin antisense and DN mutant replacing the cysteine residue at amino acid 84 with alanine (Cys84Ala) were prepared and introduced into BCG-823 and MKN-45 gastric cancer cells to establish stable transfectants. We showed that both antisense and DN mutant stable transfectants exhibited abnormal morphology, with decreased cell growth and increased rate of spontaneous apoptosis and mitotic catastrophe. Furthermore, in nude mice xenografts, these cells exhibited decreased de novo gastric tumor formation and reduced development of angiogenesis. Results from these studies strongly suggest that survivin is a promising target for gastric cancer treatment. |
| 巻・号 | 63(22) |
| ページ | 7724-32 |
| 公開日 | 2003-11-15 |
| PMID | 14633697 |
| MeSH | Adenocarcinoma / blood supply Adenocarcinoma / genetics Adenocarcinoma / pathology Adenocarcinoma / therapy* Animals Apoptosis / genetics Cell Division / genetics Cell Line, Tumor DNA, Antisense / genetics* Female Humans Inhibitor of Apoptosis Proteins Mice Mice, Inbred BALB C Mice, Nude Microtubule-Associated Proteins / antagonists & inhibitors* Microtubule-Associated Proteins / biosynthesis Microtubule-Associated Proteins / genetics* Neoplasm Proteins Neovascularization, Pathologic / genetics Neovascularization, Pathologic / therapy* Stomach Neoplasms / blood supply Stomach Neoplasms / genetics Stomach Neoplasms / pathology Stomach Neoplasms / therapy* Survivin Transfection Xenograft Model Antitumor Assays |
| IF | 9.727 |
| 引用数 | 137 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |