論文 - 詳細
| RRC ID | 4205 |
|---|---|
| 著者 | Han HF, Beckerle MC. |
| タイトル | The ALP-Enigma protein ALP-1 functions in actin filament organization to promote muscle structural integrity in Caenorhabditis elegans. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Mutations that affect the Z-disk-associated ALP-Enigma proteins have been linked to human muscular and cardiac diseases. Despite their clear physiological significance for human health, the mechanism of action of ALP-Enigma proteins is largely unknown. In Caenorhabditis elegans, the ALP-Enigma protein family is encoded by a single gene, alp-1; thus C. elegans provides an excellent model to study ALP-Enigma function. Here we present a molecular and genetic analysis of ALP-Enigma function in C. elegans. We show that ALP-1 and alpha-actinin colocalize at dense bodies where actin filaments are anchored and that the proper localization of ALP-1 at dense bodies is dependent on alpha-actinin. Our analysis of alp-1 mutants demonstrates that ALP-1 functions to maintain actin filament organization and participates in muscle stabilization during contraction. Reducing alpha-actinin activity enhances the actin filament phenotype of the alp-1 mutants, suggesting that ALP-1 and alpha-actinin function in the same cellular process. Like alpha-actinin, alp-1 also interacts genetically with a connectin/titin family member, ketn-1, to provide mechanical stability for supporting body wall muscle contraction. Taken together, our data demonstrate that ALP-1 and alpha-actinin function together to stabilize actin filaments and promote muscle structural integrity. |
| 巻・号 | 20(9) |
| ページ | 2361-70 |
| 公開日 | 2009-5-1 |
| DOI | 10.1091/mbc.e08-06-0584 |
| PII | E08-06-0584 |
| PMID | 19261811 |
| PMC | PMC2675616 |
| MeSH | Actin Cytoskeleton / metabolism* Actinin / metabolism Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Antibody Specificity Biomechanical Phenomena Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cellular Structures / metabolism Connectin LIM Domain Proteins Microfilament Proteins / metabolism Muscle Proteins / metabolism Muscles / cytology Muscles / metabolism* Mutant Proteins / metabolism Mutation / genetics Protein Transport Vinculin / metabolism |
| IF | 3.791 |
| 引用数 | 23 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1137 |