RRC ID 4206
Author Adamo A, Montemauri P, Silva N, Ward JD, Boulton SJ, La Volpe A.
Title BRC-1 acts in the inter-sister pathway of meiotic double-strand break repair.
Journal EMBO Rep.
Abstract The breast and ovarian cancer susceptibility protein BRCA1 is evolutionarily conserved and functions in DNA double-strand break (DSB) repair through homologous recombination, but its role in meiosis is poorly understood. By using genetic analysis, we investigated the role of the Caenorhabditis elegans BRCA1 orthologue (brc-1) during meiotic prophase. The null mutant in the brc-1 gene is viable, fertile and shows the wild-type complement of six bivalents in most diakinetic nuclei, which is indicative of successful crossover recombination. However, brc-1 mutants show an abnormal increase in apoptosis and RAD-51 foci at pachytene that are abolished by loss of spo-11 function, suggesting a defect in meiosis rather than during premeiotic DNA replication. In genetic backgrounds in which chiasma formation is abrogated, such as him-14/MSH4 and syp-2, loss of brc-1 leads to chromosome fragmentation suggesting that brc-1 is dispensable for crossing over but essential for DSB repair through inter-sister recombination.
Volume 9(3)
Pages 287-92
Published 2008-3
DOI 10.1038/sj.embor.7401167
PII 7401167
PMID 18219312
PMC PMC2267377
MeSH Animals Apoptosis Caenorhabditis elegans / cytology* Caenorhabditis elegans / enzymology Caenorhabditis elegans Proteins / metabolism* Crossing Over, Genetic* DNA Breaks, Double-Stranded* DNA Repair* Embryo Loss Endodeoxyribonucleases Esterases / metabolism Indoles Meiosis* Meiotic Prophase I Mutation / genetics Rad51 Recombinase / metabolism
IF 8.383
Times Cited 32
C.elegans tm1145