論文 - 詳細
| RRC ID | 4206 |
|---|---|
| 著者 | Adamo A, Montemauri P, Silva N, Ward JD, Boulton SJ, La Volpe A. |
| タイトル | BRC-1 acts in the inter-sister pathway of meiotic double-strand break repair. |
| ジャーナル | EMBO Rep |
| Abstract |
The breast and ovarian cancer susceptibility protein BRCA1 is evolutionarily conserved and functions in DNA double-strand break (DSB) repair through homologous recombination, but its role in meiosis is poorly understood. By using genetic analysis, we investigated the role of the Caenorhabditis elegans BRCA1 orthologue (brc-1) during meiotic prophase. The null mutant in the brc-1 gene is viable, fertile and shows the wild-type complement of six bivalents in most diakinetic nuclei, which is indicative of successful crossover recombination. However, brc-1 mutants show an abnormal increase in apoptosis and RAD-51 foci at pachytene that are abolished by loss of spo-11 function, suggesting a defect in meiosis rather than during premeiotic DNA replication. In genetic backgrounds in which chiasma formation is abrogated, such as him-14/MSH4 and syp-2, loss of brc-1 leads to chromosome fragmentation suggesting that brc-1 is dispensable for crossing over but essential for DSB repair through inter-sister recombination. |
| 巻・号 | 9(3) |
| ページ | 287-92 |
| 公開日 | 2008-3-1 |
| DOI | 10.1038/sj.embor.7401167 |
| PII | 7401167 |
| PMID | 18219312 |
| PMC | PMC2267377 |
| MeSH | Animals Apoptosis Caenorhabditis elegans / cytology* Caenorhabditis elegans / enzymology Caenorhabditis elegans Proteins / metabolism* Crossing Over, Genetic* DNA Breaks, Double-Stranded* DNA Repair* Embryo Loss Endodeoxyribonucleases Esterases / metabolism Indoles Meiosis* Meiotic Prophase I Mutation / genetics Rad51 Recombinase / metabolism |
| IF | 7.497 |
| 引用数 | 49 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1145 |