論文 - 詳細
| RRC ID | 42105 |
|---|---|
| 著者 | Sugiura T, Nagano Y, Inoue T, Hirotani K. |
| タイトル | A novel mitochondrial C1-tetrahydrofolate synthetase is upregulated in human colon adenocarcinoma. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
To seek the genes involved in the development of colorectal cancer, we analyzed the microarray gene expression profiles of human normal and cancerous colon tissues using the BioExpress database platform. Through the analysis we found one gene named DKFZp586G1517 that was upregulated in colon adenocarcinomas. The full-length cDNA of the DKFZp586G1517 cloned by polymerase chain reaction (PCR) encodes a protein with 978 amino acids, which is homologous to the human cytosolic C(1)-tetrahydrofolate synthetase and contains a mitochondrial target signal at N-terminus. The gene product expressed in 293 cells was localized in mitochondria and processed at the predicted signal cleavage site, supporting the idea that DKFZp586G1517 is a novel mitochondrial C(1)-tetrahydrofolate synthetase (mtC(1)-THFS). The overexpression of mtC(1)-THFS in 293 cells stimulated the colony formation. These results suggest that mtC(1)-THFS may participate in the progression of colorectal cancer by conferring growth advantage and could be a new molecular target for cancer therapy. |
| 巻・号 | 315(1) |
| ページ | 204-11 |
| 公開日 | 2004-2-27 |
| DOI | 10.1016/j.bbrc.2004.01.035 |
| PII | S0006291X04000506 |
| PMID | 15013446 |
| MeSH | Adenocarcinoma / enzymology* Amino Acid Sequence Aminohydrolases / biosynthesis* Aminohydrolases / chemistry Aminohydrolases / genetics Cell Division / genetics Cell Line Cloning, Molecular Colonic Neoplasms / enzymology* DNA, Complementary / genetics Formate-Tetrahydrofolate Ligase / biosynthesis* Formate-Tetrahydrofolate Ligase / chemistry Formate-Tetrahydrofolate Ligase / genetics Gene Expression Profiling Humans Methylenetetrahydrofolate Dehydrogenase (NADP) / biosynthesis* Methylenetetrahydrofolate Dehydrogenase (NADP) / chemistry Methylenetetrahydrofolate Dehydrogenase (NADP) / genetics Mitochondria / enzymology* Molecular Sequence Data Multienzyme Complexes / biosynthesis* Multienzyme Complexes / chemistry Multienzyme Complexes / genetics Protein Structure, Tertiary Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid Transfection Up-Regulation |
| IF | 2.985 |
| 引用数 | 28 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |