| RRC ID |
42105
|
| 著者 |
Sugiura T, Nagano Y, Inoue T, Hirotani K.
|
| タイトル |
A novel mitochondrial C1-tetrahydrofolate synthetase is upregulated in human colon adenocarcinoma.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
To seek the genes involved in the development of colorectal cancer, we analyzed the microarray gene expression profiles of human normal and cancerous colon tissues using the BioExpress database platform. Through the analysis we found one gene named DKFZp586G1517 that was upregulated in colon adenocarcinomas. The full-length cDNA of the DKFZp586G1517 cloned by polymerase chain reaction (PCR) encodes a protein with 978 amino acids, which is homologous to the human cytosolic C(1)-tetrahydrofolate synthetase and contains a mitochondrial target signal at N-terminus. The gene product expressed in 293 cells was localized in mitochondria and processed at the predicted signal cleavage site, supporting the idea that DKFZp586G1517 is a novel mitochondrial C(1)-tetrahydrofolate synthetase (mtC(1)-THFS). The overexpression of mtC(1)-THFS in 293 cells stimulated the colony formation. These results suggest that mtC(1)-THFS may participate in the progression of colorectal cancer by conferring growth advantage and could be a new molecular target for cancer therapy.
|
| 巻・号 |
315(1)
|
| ページ |
204-11
|
| 公開日 |
2004-2-27
|
| DOI |
10.1016/j.bbrc.2004.01.035
|
| PII |
S0006291X04000506
|
| PMID |
15013446
|
| MeSH |
Adenocarcinoma / enzymology*
Amino Acid Sequence
Aminohydrolases / biosynthesis*
Aminohydrolases / chemistry
Aminohydrolases / genetics
Cell Division / genetics
Cell Line
Cloning, Molecular
Colonic Neoplasms / enzymology*
DNA, Complementary / genetics
Formate-Tetrahydrofolate Ligase / biosynthesis*
Formate-Tetrahydrofolate Ligase / chemistry
Formate-Tetrahydrofolate Ligase / genetics
Gene Expression Profiling
Humans
Methylenetetrahydrofolate Dehydrogenase (NADP) / biosynthesis*
Methylenetetrahydrofolate Dehydrogenase (NADP) / chemistry
Methylenetetrahydrofolate Dehydrogenase (NADP) / genetics
Mitochondria / enzymology*
Molecular Sequence Data
Multienzyme Complexes / biosynthesis*
Multienzyme Complexes / chemistry
Multienzyme Complexes / genetics
Protein Structure, Tertiary
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Transfection
Up-Regulation
|
| IF |
2.985
|
| 引用数 |
28
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
293(RCB1637) |
