| RRC ID |
42116
|
| Author |
Ishii T, Ohshima S, Ishida T, Mima T, Tabunoki Y, Kobayashi H, Maeda M, Uede T, Liaw L, Kinoshita N, Kawase I, Saeki Y.
|
| Title |
Osteopontin as a positive regulator in the osteoclastogenesis of arthritis.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
We examined the role of osteopontin (OPN) in the osteoclastogenesis of arthritis using collagen-induced arthritis (CIA). Cells from arthritic joints of wild-type (OPN +/+) mice spontaneously developed bone-resorbing osteoclast-like cells (OCLs). The cultured cells showed an enhanced expression of receptor activator of nuclear factor kappaB ligand (RANKL) and a decreased expression of osteoprotegerin (OPG). The addition of OPG reduced the number of OCLs, indicating that the osteoclastogenesis depends on the RANK/RANKL/OPG system. The cells also produced OPN abundantly and anti-OPN neutralizing antibodies suppressed the development of OCLs. Moreover, the addition of OPN increased the expression of RANKL and augmented differentiation of OCLs from OPN-deficient (OPN -/-) cells. OPN, like the combination of 1alpha,25-dihydroxyvitamin D(3) and dexamethasone, also enhanced the RANKL expression and decreased OPG expression in a stromal cell line, ST2. These results suggest that OPN acts as a positive regulator in the osteoclastogenesis of arthritis through the RANK/RANKL/OPG system.
|
| Volume |
316(3)
|
| Pages |
809-15
|
| Published |
2004-4-9
|
| DOI |
10.1016/j.bbrc.2004.02.124
|
| PII |
S0006291X0400395X
|
| PMID |
15033472
|
| MeSH |
Animals
Arthritis / etiology
Arthritis / metabolism*
Calcitriol / metabolism
Carrier Proteins / metabolism
Cell Differentiation
Cell Line
Cells, Cultured
Collagen / metabolism
Dexamethasone / pharmacology
Dose-Response Relationship, Drug
Gene Expression Regulation*
Glycoproteins / metabolism
Membrane Glycoproteins / metabolism
Mice
Mice, Inbred DBA
Mice, Transgenic
Models, Genetic
Osteoclasts / metabolism
Osteopontin
Osteoprotegerin
RANK Ligand
RNA, Messenger / metabolism
Receptor Activator of Nuclear Factor-kappa B
Receptors, Cytoplasmic and Nuclear / metabolism
Receptors, Tumor Necrosis Factor
Recombinant Proteins / chemistry
Reverse Transcriptase Polymerase Chain Reaction
Sialoglycoproteins / physiology*
Stromal Cells / cytology
|
| IF |
2.985
|
| Times Cited |
38
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
ST2(RCB0224) |
