論文 - 詳細
| RRC ID | 4220 |
|---|---|
| 著者 | de Carvalho CE, Zaaijer S, Smolikov S, Gu Y, Schumacher JM, Colaiácovo MP. |
| タイトル | LAB-1 antagonizes the Aurora B kinase in C. elegans. |
| ジャーナル | Genes Dev |
| Abstract |
The Shugoshin/Aurora circuitry that controls the timely release of cohesins from sister chromatids in meiosis and mitosis is widely conserved among eukaryotes, although little is known about its function in organisms whose chromosomes lack a localized centromere. Here we show that Caenorhabditis elegans chromosomes rely on an alternative mechanism to protect meiotic cohesin that is shugoshin-independent and instead involves the activity of a new chromosome-associated protein named LAB-1 (Long Arm of the Bivalent). LAB-1 preserves meiotic sister chromatid cohesion by restricting the localization of the C. elegans Aurora B kinase, AIR-2, to the interface between homologs via the activity of the PP1/Glc7 phosphatase GSP-2. The localization of LAB-1 to chromosomes of dividing embryos and the suppression of mitotic-specific defects in air-2 mutant embryos with reduced LAB-1 activity support a global role of LAB-1 in antagonizing AIR-2 in both meiosis and mitosis. Although the localization of a GFP fusion and the analysis of mutants and RNAi-mediated knockdowns downplay a role for the C. elegans shugoshin protein in cohesin protection, shugoshin nevertheless helps to ensure the high fidelity of chromosome segregation at metaphase I. We propose that, in C. elegans, a LAB-1-mediated mechanism evolved to offset the challenges of providing protection against separase activity throughout a larger chromosome area. |
| 巻・号 | 22(20) |
| ページ | 2869-85 |
| 公開日 | 2008-10-15 |
| DOI | 10.1101/gad.1691208 |
| PII | 22/20/2869 |
| PMID | 18923084 |
| PMC | PMC2569883 |
| MeSH | Adenosine Triphosphatases / metabolism Amino Acid Sequence Animals Aurora Kinase B Aurora Kinases Caenorhabditis elegans Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / metabolism Chromatids / genetics Chromatids / metabolism Chromosomal Proteins, Non-Histone / antagonists & inhibitors Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Chromosome Segregation Cohesins DNA-Binding Proteins / metabolism Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Fluorescent Antibody Technique Gene Expression Regulation, Developmental* Immunoglobulin G / immunology Meiosis / physiology Meiotic Prophase I / physiology Mitosis / physiology Molecular Sequence Data Multiprotein Complexes / metabolism Protein Serine-Threonine Kinases / antagonists & inhibitors* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism RNA, Helminth / genetics RNA, Helminth / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / pharmacology Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sister Chromatid Exchange |
| IF | 9.527 |
| 引用数 | 61 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1791 tm2443 tm2344 tm301 tm3002 |