論文 - 詳細
| RRC ID | 42233 |
|---|---|
| 著者 | Isnardi I, Lesourne R, Bruhns P, Fridman WH, Cambier JC, Daëron M. |
| タイトル | Two distinct tyrosine-based motifs enable the inhibitory receptor FcgammaRIIB to cooperatively recruit the inositol phosphatases SHIP1/2 and the adapters Grb2/Grap. |
| ジャーナル | J Biol Chem |
| Abstract |
FcgammaRIIB are low-affinity receptors for IgG that contain an immunoreceptor tyrosine-based inhibition motif (ITIM) and inhibit immunoreceptor tyrosine-based activation motif (ITAM)-dependent cell activation. When coaggregated with ITAM-bearing receptors, FcgammaRIIB become tyrosyl-phosphorylated and recruit the Src homology 2 (SH2) domain-containing inositol 5'-phosphatases SHIP1 and SHIP2, which mediate inhibition. The FcgammaRIIB ITIM was proposed to be necessary and sufficient for recruiting SHIP1/2. We show here that a second tyrosine-containing motif in the intracytoplasmic domain of FcgammaRIIB is required for SHIP1/2 to be coprecipitated with the receptor. This motif functions as a docking site for the SH2 domain-containing adapters Grb2 and Grap. These adapters interact via their C-terminal SH3 domain with SHIP1/2 to form a stable receptor-phosphatase-adapter trimolecular complex. Both Grb2 and Grap are required for an optimal coprecipitation of SHIP with FcgammaRIIB, but one adapter is sufficient for the phosphatase to coprecipitate in a detectable manner with the receptors. In addition to facilitating the recruitment of SHIPs, the second tyrosine-based motif may confer upon FcgammaRIIB the properties of scaffold proteins capable of altering the composition and stability of the signaling complexes generated following receptor engagement. |
| 巻・号 | 279(50) |
| ページ | 51931-8 |
| 公開日 | 2004-12-10 |
| DOI | 10.1074/jbc.M410261200 |
| PII | S0021-9258(20)67628-1 |
| PMID | 15456754 |
| MeSH | Adaptor Proteins, Signal Transducing / chemistry Adaptor Proteins, Signal Transducing / metabolism* Amino Acid Motifs Amino Acid Sequence Animals Binding Sites Cell Line Chickens GRB2 Adaptor Protein In Vitro Techniques Inositol Polyphosphate 5-Phosphatases Mice Molecular Sequence Data Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases Phosphoric Monoester Hydrolases / chemistry Phosphoric Monoester Hydrolases / metabolism* Protein Binding Receptors, IgG / chemistry* Receptors, IgG / genetics Receptors, IgG / metabolism* Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Transfection Tyrosine / chemistry src Homology Domains |
| IF | 4.238 |
| 引用数 | 37 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |