RRC ID 42258
Author Meng H, Lu C, Mabuchi H, Tanigawa N.
Title Prognostic significance and different properties of survivin splicing variants in gastric cancer.
Journal Cancer Lett
Abstract Survivin, a novel antiapoptosis gene, was identified as a member of the inhibitor of apoptosis protein (IAP) family. Unique among IAP, survivin has been found to be abundantly expressed in a wide variety of human malignancies, whereas it is undetectable in normal adult tissues. Recently, three splicing variants of survivin have been further characterized with different subcellular localization, but their different functions in carcinogenesis are largely unknown. We used real time quantitative RT-PCR to analyse survivin variants' mRNA expression levels in 77 gastric carcinoma cases whose frozen samples were available. All the cases and seven cell lines tested expressed wild-type survivin mRNA, which was not only the dominant transcript, but also was a poor prognostic biomarker (P = 0.003). Non-antiapoptosis survivin-2B mRNA was negatively correlated with tumor stage (P = 0.001) histological type (P = 0.007) and depth of tumor invasion (P = 0.031) while survivin-DeltaEx3 mRNA showed a significant reverse association with apoptosis ( P = 0.019). These data demonstrated that survivin mRNA expression levels are of important prognostic value, suggesting the significant participation of survivin-2B and survivin-DeltaEx3 in gastric cancer development.
Volume 216(2)
Pages 147-55
Published 2004-12-28
DOI 10.1016/j.canlet.2003.12.020
PII S0304383504000242
PMID 15533590
MeSH Adult Aged Aged, 80 and over Alternative Splicing Antigens, Neoplasm / genetics Apoptosis* Cell Line, Tumor Female Gene Expression Regulation, Neoplastic* Humans Immunohistochemistry Inhibitor of Apoptosis Proteins Ki-67 Antigen / analysis Male Microtubule-Associated Proteins / biosynthesis Microtubule-Associated Proteins / genetics* Middle Aged Neoplasm Proteins Postoperative Period Prognosis Protein Isoforms / biosynthesis Protein Isoforms / genetics RNA, Messenger / analysis Stomach Neoplasms / genetics* Stomach Neoplasms / metabolism Stomach Neoplasms / pathology Survivin
IF 7.36
Times Cited 44
Human and Animal Cells