| RRC ID |
42351
|
| Author |
Okajima S, Sakai Y, Yamaguchi T.
|
| Title |
Development of a regenerable cell culture system that senses and releases dead cells.
|
| Journal |
Langmuir
|
| Abstract |
We developed a rapidly regenerable cell culture system in which the cell culture substrate detects cell death and selectively releases the dead cells. This culture material was achieved by combining a detector that responds to the signal from the dead cells and an actuator to release the dead cells. Benzo-18-crown-6-acrylamide (BCAm) with a pendant crown ether receptor was used as the sensor to recognize cellular signals and N-isopropylacrylamide (NIPAM) was used as the actuator. This copolymer of NIPAM and BCAm can respond to potassium ions and change its nature from hydrophobic to hydrophilic at the culture temperature of 37 degrees C. Living cells concentrate potassium ion internally; when cells die, potassium ions are released. The polymer surface recognizes the potassium ions released from the dead cells, the NIPAM hydrates, and the dead cells are selectively detached. This in vitro culture system is a novel one in which artificial culture materials work cooperatively with cellular metabolism by responding to this signal from the cells, thereby realizing in vitro tissue regeneration partly mimicking the mechanisms of in vivo homeostasis.
|
| Volume |
21(9)
|
| Pages |
4043-9
|
| Published |
2005-4-26
|
| DOI |
10.1021/la046994e
|
| PMID |
15835972
|
| MeSH |
Acrylamides / chemistry*
Biosensing Techniques
Cations / chemistry
Cell Culture Techniques / methods*
Cell Death
Cell Membrane / metabolism
Cells, Cultured
Crown Ethers / chemistry*
Epithelial Cells
Homeostasis
Humans
Hydrophobic and Hydrophilic Interactions
Potassium / chemistry
Potassium / metabolism
Temperature
Time Factors
Tissue Engineering / methods*
|
| IF |
3.557
|
| Times Cited |
42
|
|
WOS Category
|
MATERIALS SCIENCE, MULTIDISCIPLINARY
CHEMISTRY, PHYSICAL
CHEMISTRY, MULTIDISCIPLINARY
|
| Resource |
| Human and Animal Cells |
A549(RCB0098) |
