| RRC ID |
42360
|
| 著者 |
Hamaguchi T, Matsuoka Y, Bechberger J, Ohnishi T, Fujita K, Naus CC, Kusunoki M, Tsubura A, Tsuda H.
|
| タイトル |
Establishment of an apoptosis-sensitive rat mammary carcinoma cell line with a mutation in the DNA-binding region of p53.
|
| ジャーナル |
Cancer Lett
|
| Abstract |
Seven mammary carcinoma cell lines were established from 7,12-dimethylbenz[a]anthracene-induced tumors developed in a human c-Ha-ras transgenic rat. Without apoptotic stimuli, a large amount of p53 protein was detected in the C11 cell line (C11), whereas all cell lines expressed variable levels of the assayed death receptor/ligand, bcl-2 family and p53 cascade-related genes. The p53 gene in C11 had a mutation at codon 246, in the DNA-binding region of p53. Transcriptional activity of the mutant protein appeared to be lower than that of the wild-type p53. Despite the presence of p53 mutation, C11 was more sensitive to apoptosis triggered by etoposide, paclitaxel and staurosporine than the cell lines expressing wild-type p53. These data suggest that the apoptosis induced by intracellular injury occurs via the transcriptionally impaired mutant p53 in C11.
|
| 巻・号 |
232(2)
|
| ページ |
279-88
|
| 公開日 |
2006-2-8
|
| DOI |
10.1016/j.canlet.2005.02.043
|
| PII |
S0304-3835(05)00233-8
|
| PMID |
15876486
|
| MeSH |
Animals
Apoptosis*
Binding Sites
Cell Line, Tumor
DNA / metabolism*
Female
Genes, ras
Humans
MAP Kinase Signaling System
Mammary Neoplasms, Experimental / genetics*
Mammary Neoplasms, Experimental / pathology
Point Mutation*
Rats
Tumor Suppressor Protein p53 / genetics*
Tumor Suppressor Protein p53 / metabolism
bcl-X Protein / metabolism
|
| IF |
7.36
|
| 引用数 |
7
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RCN-9(RCB0511) |
