RRC ID 42397
Author Nanmoku T, Takekoshi K, Fukuda T, Ishii K, Isobe K, Kawakami Y.
Title Stimulation of catecholamine biosynthesis via the PKC pathway by prolactin-releasing peptide in PC12 rat pheochromocytoma cells.
Journal J Endocrinol
Abstract We have previously shown that prolactin-releasing peptide (PrRP) stimulates catecholamine release from PC12 cells (rat pheochromocytoma cell line). However, it is not known whether PrRP also affects catecholamine biosynthesis. Thus, we examined the effect of PrRP on catecholamine biosynthesis in PC12 cells. PrRP31 (>10 nM) and PrRP20 (>100 nM) significantly increased the activity and expression level of tyrosine hydroxylase (TH), a rate-limiting enzyme, in catecholamine biosynthesis. However, the PrRP20-stimulated TH activity was markedly weaker than that of PrRP31. PrRP31 (>1 nM) and PrRP20 (>10 nM) significantly induced an increase in the level of PKC activity. Both Ro 32-0432 (a protein kinase C inhibitor) and H89 (a protein kinase A inhibitor) effectively suppressed the PrRP31 (100 nM)-induced TH mRNA level. Next, we examined the effect of PrRP on mitogen-activated protein kinases (MAPKs). PrRP31 (1 microM) significantly induced an increase in the activity of extracellular signal-related kinases (ERKs) and the stress-activated protein kinase/c-jun N terminal kinase (SAPK/JNK). In contrast to ERKs and JNK, PrRP31 did not affect P38 MAPK activity. Consistent with these findings, pretreatment of cells with the MEK-1-inhibitor, PD-98059 (50 microM), significantly inhibited the PrRP31 (100 nM)-induced increase in TH mRNA. These results indicate that PrRP stimulates catecholamine synthesis through both the PKC and PKA pathways in PC12 cells.
Volume 186(1)
Pages 233-9
Published 2005-7-1
DOI 10.1677/joe.1.05919
PII 186/1/233
PMID 16002552
MeSH Animals Catecholamines / biosynthesis* Cell Line, Tumor Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors Cyclic AMP-Dependent Protein Kinases / metabolism Flavonoids / pharmacology Hypothalamic Hormones / pharmacology* Indoles / pharmacology Isoquinolines / pharmacology MAP Kinase Signaling System / drug effects Neuropeptides / pharmacology* Pheochromocytoma / metabolism* Prolactin-Releasing Hormone Protein Kinase C / antagonists & inhibitors Protein Kinase C / metabolism* Pyrroles / pharmacology RNA, Messenger / analysis Rats Signal Transduction / drug effects* Stimulation, Chemical Sulfonamides / pharmacology Tyrosine 3-Monooxygenase / genetics Tyrosine 3-Monooxygenase / metabolism*
IF 4.041
Times Cited 15
Human and Animal Cells PC-12(RCB0009)