RRC ID 42509
著者 Takeda K, Yokoyama S, Aburatani H, Masuda T, Han F, Yoshizawa M, Yamaki N, Yamamoto H, Eguchi N, Urade Y, Shibahara S.
タイトル Lipocalin-type prostaglandin D synthase as a melanocyte marker regulated by MITF.
ジャーナル Biochem Biophys Res Commun
Abstract Microphthalmia-associated transcription factor (MITF) is responsible for differentiation of melanocytes. A recessive MITF mutant, black-eyed white Mitf(mi-bw) mouse, is characterized by white coat color and deafness, due to the lack of melanocytes in the skin and inner ears. By cDNA microarray analysis, we have identified lipocalin-type prostaglandin D synthase (L-PGDS), whose mRNA is undetectable in the homozygous Mitf(mi-bw) skin. Immunohistochemical analysis of wild-type mice identified the specific expression of L-PGDS in follicular melanocytes. L-PGDS mRNA is expressed in B16 mouse melanoma cells, but undetectable in human melanoma cell lines. RNA interference analysis against MITF suggests that L-PGDS expression is dependent on MITF in B16 melanoma cells. Furthermore, we have provided evidence that MITF is involved in the melanocyte lineage-specific transcription of the mouse L-PGDS gene. Thus, L-PGDS represents a newly identified melanocyte marker. MITF may modulate the production of prostaglandin D(2) by activating the L-PGDS gene in melanocytes.
巻・号 339(4)
ページ 1098-106
公開日 2006-1-27
DOI 10.1016/j.bbrc.2005.11.125
PII S0006-291X(05)02629-X
PMID 16337607
MeSH Animals Biomarkers / metabolism Biomarkers, Tumor / metabolism* Cells, Cultured Gene Expression Profiling Intramolecular Oxidoreductases / metabolism* Lipocalins Melanocytes / metabolism* Melanoma / metabolism* Mice Mice, Inbred BALB C Mice, Inbred C3H Microphthalmia-Associated Transcription Factor / metabolism* Retinal Ganglion Cells / metabolism* Skin / metabolism* Tissue Distribution
IF 2.985
引用数 26
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HMV-II(RCB0777)