| RRC ID |
42661
|
| 著者 |
Nakano N, Fukuhara-Takaki K, Jono T, Nakajou K, Eto N, Horiuchi S, Takeya M, Nagai R.
|
| タイトル |
Association of advanced glycation end products with A549 cells, a human pulmonary epithelial cell line, is mediated by a receptor distinct from the scavenger receptor family and RAGE.
|
| ジャーナル |
J Biochem
|
| Abstract |
Cellular interactions with advanced glycation end products (AGE)-modified proteins are known to induce several biological responses, not only endocytic uptake and degradation, but also the induction of cytokines and growth factors, combined responses that may be linked to the development of diabetic vascular complications. In this study we demonstrate that A549 cells, a human pulmonary epithelial cell line, possess a specific binding site for AGE-modified bovine serum albumin (AGE-BSA) (K(d) = 27.8 nM), and additionally for EN-RAGE (extracellular newly identified RAGE binding protein) (K(d) = 118 nM). Western blot and RT-PCR analysis showed that RAGE (receptor for AGE) is highly expressed on A549 cells, while the expression of other known AGE-receptors such as galectin-3 and SR-A (class A scavenger receptor), are below the level of detection. The binding of (125)I-AGE-BSA to these cells is inhibited by unlabeled AGE-BSA, but not by EN-RAGE. In contrast, the binding of (125)I-EN-RAGE is significantly inhibited by unlabeled EN-RAGE and soluble RAGE, but not by AGE-BSA. Our results indicate that A549 cells possess at least two binding sites, one specific for EN-RAGE and the other specific for AGE-BSA. The latter receptor on A549 cells is distinct from the scavenger receptor family and RAGE.
|
| 巻・号 |
139(5)
|
| ページ |
821-9
|
| 公開日 |
2006-5-1
|
| DOI |
10.1093/jb/mvj092
|
| PII |
139/5/821
|
| PMID |
16751589
|
| MeSH |
Animals
Cattle
Cells, Cultured
Epithelial Cells / metabolism*
Glycation End Products, Advanced / metabolism*
Glycation End Products, Advanced / pharmacokinetics
Humans
Lung / metabolism
Protein Binding
Receptor for Advanced Glycation End Products
Receptors, Immunologic / agonists
Receptors, Immunologic / classification*
Receptors, Scavenger / agonists
Receptors, Scavenger / classification
Receptors, Scavenger / metabolism
Recombinant Proteins / metabolism
Serum Albumin, Bovine / metabolism
Serum Albumin, Bovine / pharmacokinetics
Signal Transduction
Substrate Specificity
|
| IF |
2.476
|
| 引用数 |
18
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098) |
