論文 - 詳細
| RRC ID | 42744 |
|---|---|
| 著者 | Ando T, Ichikawa J, Wako M, Hatsushika K, Watanabe Y, Sakuma M, Tasaka K, Ogawa H, Hamada Y, Yagita H, Nakao A. |
| タイトル | TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells. |
| ジャーナル | Arthritis Res Ther |
| Abstract |
Tumour necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK), a member of the TNF family, is a multifunctional cytokine that regulates cell growth, migration, and survival principally through a TWEAK receptor, fibroblast growth factor-inducible 14 (Fn14). However, its physiological roles in bone are largely unknown. We herein report various effects of TWEAK on mouse osteoblastic MC3T3-E1 cells. MC3T3-E1 cells expressed Fn14 and produced RANTES (regulated upon activation, healthy T cell expressed and secreted) upon TWEAK stimulation through PI3K-Akt, but not nuclear factor-kappaB (NF-kappaB), pathway. In addition, TWEAK inhibited bone morphogenetic protein (BMP)-2-induced expression of osteoblast differentiation markers such as alkaline phosphatase through mitogen-activated protein kinase (MAPK) Erk pathway. Furthermore, TWEAK upregulated RANKL (receptor activation of NF-kappaB ligand) expression through MAPK Erk pathway in MC3T3-E1 cells. All these effects of TWEAK on MC3T3-E1 cells were abolished by mouse Fn14-Fc chimera. We also found significant TWEAK mRNA or protein expression in osteoblast- and osteoclast-lineage cell lines or the mouse bone tissue, respectively. Finally, we showed that human osteoblasts expressed Fn14 and induced RANTES and RANKL upon TWEAK stimulation. Collectively, TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in MC3T3-E1 cells. TWEAK may thus be a novel cytokine that regulates several aspects of osteoblast function. |
| 巻・号 | 8(5) |
| ページ | R146 |
| 公開日 | 2006-1-1 |
| DOI | 10.1186/ar2038 |
| PII | ar2038 |
| PMID | 16945157 |
| PMC | PMC1779446 |
| MeSH | 3T3 Cells Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / pharmacology* Cell Differentiation / drug effects Chemokine CCL5 / metabolism* Chimera Cytokine TWEAK Gene Expression / drug effects Gene Expression / physiology Humans Mice Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / metabolism* Phosphatidylinositol 3-Kinases / metabolism Proto-Oncogene Proteins c-akt / metabolism RANK Ligand / genetics* RNA, Messenger / metabolism Receptors, Tumor Necrosis Factor / genetics Receptors, Tumor Necrosis Factor / metabolism* Recombinant Proteins / genetics Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Signal Transduction / drug effects Signal Transduction / physiology TWEAK Receptor Transforming Growth Factor beta / pharmacology* Tumor Necrosis Factors / genetics Tumor Necrosis Factors / metabolism* Tumor Necrosis Factors / pharmacology |
| IF | 4.103 |
| 引用数 | 52 |
| WOS 分野 | RHEUMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) RAW 264(RCB0535) ATDC5(RCB0565) EL4(RCB1641) |