RRC ID 42789
Author Li HM, Taira T, Maita C, Ariga H, Iguchi-Ariga SM.
Title Protection against nonylphenol-induced cell death by DJ-1 in cultured Japanese medaka (Oryzias latipes) cells.
Journal Toxicology
Abstract The Japanese medaka (Oryzias latipes) has been used to investigate diverse aspects of toxicology, genetics and developmental biology and to monitor biological changes caused by endocrine disruptors. In this study, we analyzed a medaka homolog of human DJ-1 (meDJ-1) in cultured medaka cells into which nonylphenol (NP) was added. Like human DJ-1, meDJ-1 was found to be oxidized by treatment with H(2)O(2) and its pI was shifted to acidic points. NP was found to induce cell death with kinetics similar to that of H(2)O(2) in cultured medaka OLHE-13 cells. After OLHE-13 cells had been treated with sub-lethal concentrations of H(2)O(2) and NP, production of reactive oxygen species and oxidation of meDJ-1 were observed. meDJ-1 knockdown by short interfering RNA rendered OLHE-13 cells susceptible to H(2)O(2) and NP-induced cell death, suggesting a protective role of DJ-1 against oxidative stress-induced cell death in medaka cells. These results suggest that meDJ-1 is a suitable biomarker for oxidative stress reactions in medaka.
Volume 228(2-3)
Pages 229-38
Published 2006-12-7
DOI 10.1016/j.tox.2006.08.040
PII S0300-483X(06)00550-6
PMID 17034925
MeSH Amino Acid Sequence Animals Biomarkers Blotting, Western Cell Death / drug effects* Cell Survival Cells, Cultured DNA, Complementary / biosynthesis DNA, Complementary / genetics Flow Cytometry Intracellular Signaling Peptides and Proteins / pharmacology* Isoelectric Focusing Luciferases / metabolism Molecular Sequence Data Oncogene Proteins / pharmacology* Oryzias / physiology* Oxidation-Reduction Oxidative Stress / drug effects Phenols / antagonists & inhibitors* Phenols / toxicity* Protein Deglycase DJ-1 Reverse Transcriptase Polymerase Chain Reaction Transfection
IF 3.547
Times Cited 11
Human and Animal Cells