| RRC ID |
42789
|
| 著者 |
Li HM, Taira T, Maita C, Ariga H, Iguchi-Ariga SM.
|
| タイトル |
Protection against nonylphenol-induced cell death by DJ-1 in cultured Japanese medaka (Oryzias latipes) cells.
|
| ジャーナル |
Toxicology
|
| Abstract |
The Japanese medaka (Oryzias latipes) has been used to investigate diverse aspects of toxicology, genetics and developmental biology and to monitor biological changes caused by endocrine disruptors. In this study, we analyzed a medaka homolog of human DJ-1 (meDJ-1) in cultured medaka cells into which nonylphenol (NP) was added. Like human DJ-1, meDJ-1 was found to be oxidized by treatment with H(2)O(2) and its pI was shifted to acidic points. NP was found to induce cell death with kinetics similar to that of H(2)O(2) in cultured medaka OLHE-13 cells. After OLHE-13 cells had been treated with sub-lethal concentrations of H(2)O(2) and NP, production of reactive oxygen species and oxidation of meDJ-1 were observed. meDJ-1 knockdown by short interfering RNA rendered OLHE-13 cells susceptible to H(2)O(2) and NP-induced cell death, suggesting a protective role of DJ-1 against oxidative stress-induced cell death in medaka cells. These results suggest that meDJ-1 is a suitable biomarker for oxidative stress reactions in medaka.
|
| 巻・号 |
228(2-3)
|
| ページ |
229-38
|
| 公開日 |
2006-12-7
|
| DOI |
10.1016/j.tox.2006.08.040
|
| PII |
S0300-483X(06)00550-6
|
| PMID |
17034925
|
| MeSH |
Amino Acid Sequence
Animals
Biomarkers
Blotting, Western
Cell Death / drug effects*
Cell Survival
Cells, Cultured
DNA, Complementary / biosynthesis
DNA, Complementary / genetics
Flow Cytometry
Intracellular Signaling Peptides and Proteins / pharmacology*
Isoelectric Focusing
Luciferases / metabolism
Molecular Sequence Data
Oncogene Proteins / pharmacology*
Oryzias / physiology*
Oxidation-Reduction
Oxidative Stress / drug effects
Phenols / antagonists & inhibitors*
Phenols / toxicity*
Protein Deglycase DJ-1
Reverse Transcriptase Polymerase Chain Reaction
Transfection
|
| IF |
4.099
|
| 引用数 |
13
|
|
WOS 分野
|
TOXICOLOGY
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
|
