論文 - 詳細
| RRC ID | 4280 |
|---|---|
| 著者 | Pispa J, Palmén S, Holmberg CI, Jäntti J. |
| タイトル | C. elegans dss-1 is functionally conserved and required for oogenesis and larval growth. |
| ジャーナル | BMC Dev Biol |
| Abstract |
BACKGROUND:Dss1 (or Rpn15) is a recently identified subunit of the 26S proteasome regulatory particle. In addition to its function in the protein degradation machinery, it has been linked to BRCA2 (breast cancer susceptibility gene 2 product) and homologous DNA recombination, mRNA export, and exocytosis. While the fungal orthologues of Dss1 are not essential for viability, the significance of Dss1 in metazoans has remained unknown due to a lack of knockout animal models. RESULTS:In the current study deletion of dss-1 was studied in Caenorhabditis elegans with a dss-1 loss-of-function mutant and dss-1 directed RNAi. The analysis revealed an essential role for dss-1 in oogenesis. In addition, dss-1 RNAi caused embryonic lethality and larval arrest, presumably due to loss of the dss-1 mRNA maternal contribution. DSS-1::GFP fusion protein localised primarily in the nucleus. No apparent effect on proteasome function was found in dss-1 RNAi treated worms. However, expression of the C. elegans dss-1 in yeast cells deleted for its orthologue SEM1 rescued their temperature-sensitive growth phenotype, and partially rescued the accumulation of polyubiquitinated proteins in these cells. CONCLUSION:The first knockout animal model for the gene encoding the proteasome subunit DSS-1/Rpn15/Sem1 is characterised in this study. In contrast to unicellular eukaryotes, the C. elegans dss-1 encodes an essential protein, which is required for embryogenesis, larval growth, and oogenesis, and which is functionally conserved with its yeast and human homologues. |
| 巻・号 | 8 |
| ページ | 51 |
| 公開日 | 2008-5-9 |
| DOI | 10.1186/1471-213X-8-51 |
| PII | 1471-213X-8-51 |
| PMID | 18471277 |
| PMC | PMC2409312 |
| MeSH | Animals Base Sequence Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* DNA Primers / genetics Female Fertility / genetics Fertility / physiology Gene Expression Regulation, Developmental Gene Targeting Genes, Helminth Larva / growth & development Male Mutation Oogenesis / genetics Oogenesis / physiology* Phenotype Proteasome Endopeptidase Complex / genetics Proteasome Endopeptidase Complex / physiology RNA Interference RNA, Helminth / genetics RNA, Helminth / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism |
| IF | 2.0 |
| 引用数 | 22 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| 線虫 | tm370 tm1086 tm1349 |