RRC ID 42826
Author Watatani Y, Kimura N, Shimizu YI, Akiyama I, Tonaki D, Hirose H, Takahashi S, Takahashi Y.
Title Amino acid limitation induces expression of ATF5 mRNA at the post-transcriptional level.
Journal Life Sci
Abstract ATF5 is a transcription factor in the cAMP response element (CRE)-binding protein/activating transcription factor (CREB/ATF) family. We studied the effect of amino acid limitation on ATF5 mRNA levels in a mammalian cell line. Northern-blot analysis demonstrated that limitation of a single amino acid, glutamine, methionine, or leucine, resulted in increased ATF5 mRNA levels in HeLaS3 cells. This resulted, at least in part, from increased half-life of the ATF5 mRNA transcript. Cycloheximide inhibited the increase in ATF5 mRNA expression induced by glutamine limitation, indicating that it was dependent on de novo protein synthesis. Moreover, rapamycin had no effect on basal ATF5 mRNA expression or on increased expression induced by glutamine limitation. These results indicate that amino acid limitation regulates ATF5 mRNA expression during post-transcription in a rapamycin-independent manner. The potential role for ATF5 in protecting cells from amino acid-limitation is of considerable interest.
Volume 80(9)
Pages 879-85
Published 2007-2-6
DOI 10.1016/j.lfs.2006.11.013
PII S0024-3205(06)00871-X
PMID 17140605
MeSH Activating Transcription Factors / biosynthesis* Amino Acids / deficiency* Blotting, Northern Cell Culture Techniques Cell Line, Tumor Cycloheximide / pharmacology DNA, Complementary Dactinomycin / pharmacology Humans Protein Kinase Inhibitors / pharmacology Protein Kinases / metabolism RNA Processing, Post-Transcriptional* RNA, Messenger / metabolism* Sirolimus / pharmacology TOR Serine-Threonine Kinases
IF 3.448
Times Cited 30
WOS Category MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY
Resource
Human and Animal Cells