| RRC ID |
42826
|
| 著者 |
Watatani Y, Kimura N, Shimizu YI, Akiyama I, Tonaki D, Hirose H, Takahashi S, Takahashi Y.
|
| タイトル |
Amino acid limitation induces expression of ATF5 mRNA at the post-transcriptional level.
|
| ジャーナル |
Life Sci
|
| Abstract |
ATF5 is a transcription factor in the cAMP response element (CRE)-binding protein/activating transcription factor (CREB/ATF) family. We studied the effect of amino acid limitation on ATF5 mRNA levels in a mammalian cell line. Northern-blot analysis demonstrated that limitation of a single amino acid, glutamine, methionine, or leucine, resulted in increased ATF5 mRNA levels in HeLaS3 cells. This resulted, at least in part, from increased half-life of the ATF5 mRNA transcript. Cycloheximide inhibited the increase in ATF5 mRNA expression induced by glutamine limitation, indicating that it was dependent on de novo protein synthesis. Moreover, rapamycin had no effect on basal ATF5 mRNA expression or on increased expression induced by glutamine limitation. These results indicate that amino acid limitation regulates ATF5 mRNA expression during post-transcription in a rapamycin-independent manner. The potential role for ATF5 in protecting cells from amino acid-limitation is of considerable interest.
|
| 巻・号 |
80(9)
|
| ページ |
879-85
|
| 公開日 |
2007-2-6
|
| DOI |
10.1016/j.lfs.2006.11.013
|
| PII |
S0024-3205(06)00871-X
|
| PMID |
17140605
|
| MeSH |
Activating Transcription Factors / biosynthesis*
Amino Acids / deficiency*
Blotting, Northern
Cell Culture Techniques
Cell Line, Tumor
Cycloheximide / pharmacology
DNA, Complementary
Dactinomycin / pharmacology
Humans
Protein Kinase Inhibitors / pharmacology
Protein Kinases / metabolism
RNA Processing, Post-Transcriptional*
RNA, Messenger / metabolism*
Sirolimus / pharmacology
TOR Serine-Threonine Kinases
|
| IF |
3.647
|
| 引用数 |
30
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
|
