論文 - 詳細
| RRC ID | 42996 |
|---|---|
| 著者 | Tsuboi K, Moritake T, Tsuchida Y, Tokuuye K, Matsumura A, Ando K. |
| タイトル | Cell cycle checkpoint and apoptosis induction in glioblastoma cells and fibroblasts irradiated with carbon beam. |
| ジャーナル | J Radiat Res |
| Abstract |
This study was conducted in order to evaluate the cytotoxicity of high linear-energy-transfer (LET) ionizing radiation (IR) on glioblastoma cells and fibroblasts using different modes of cell inactivation assays. Two human glioblastoma cell lines with or without p53-mutation, and fibroblasts were used as materials. Gamma rays and 290 MeV/u carbon beams with LET values of 20, 40, 80 keV/mum were used. To evaluate cell inactivation, we used colony formation assay, morphological detection of apoptosis, and flow-cytometry. Serial expressions of p53 and p21 were analyzed by immunoblotting. High-LET IR reduced the reproductive potency of these cells to identical levels in spite of differences in gamma-sensitivity, and yield of cell death correlated to LET values. A p53-wild-type glioblastoma cell line demonstrated a higher yield of apoptosis than other cell lines, whereas fibroblasts hardly displayed any cell death indicating senescence-like growth arrest even after high LET IR. A p53-mutant tumor cell line demonstrated very low yield of cell death with prominent G2/M arrest. Results of radiosensitivity differ according to what mode of cell inactivation is selected. While fibroblasts depend on G1 block after IR, G2/M blocks may play crucial roles in the radioresistance of p53-mutant glioblastoma cells. |
| 巻・号 | 48(4) |
| ページ | 317-25 |
| 公開日 | 2007-7-1 |
| DOI | 10.1269/jrr.06081 |
| PII | JST.JSTAGE/jrr/06081 |
| PMID | 17548940 |
| MeSH | Apoptosis* Carbon / chemistry* Cell Cycle / radiation effects* Cell Line, Tumor Dose-Response Relationship, Drug Fibroblasts / metabolism Fibroblasts / radiation effects* Flow Cytometry Gene Expression Regulation, Neoplastic / radiation effects* Genes, p53 Glioblastoma / radiotherapy* Humans Linear Energy Transfer Microscopy, Fluorescence Mutation Radiation, Ionizing |
| IF | 1.95 |
| 引用数 | 40 |
| WOS 分野 | RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |