論文 - 詳細
| RRC ID | 43102 |
|---|---|
| 著者 | Yonezawa D, Sekiguchi F, Miyamoto M, Taniguchi E, Honjo M, Masuko T, Nishikawa H, Kawabata A. |
| タイトル | A protective role of hydrogen sulfide against oxidative stress in rat gastric mucosal epithelium. |
| ジャーナル | Toxicology |
| Abstract |
We investigated effect of hydrogen sulfide (H(2)S) on oxidative stress-caused cell death in gastric mucosal epithelial cells. In rat normal gastric epithelial RGM1 cells, NaHS, a H(2)S donor, at 1.5mM strongly suppressed hydrogen peroxide (H(2)O(2))-caused cell death, while it slightly augmented the H(2)O(2) toxicity at 0.5-1mM. The protective effect of NaHS was abolished by inhibitors of MEK or JNK, but not of p38 MAP kinase. NaHS at 1.5mM actually phosphorylated ERK and JNK in RGM1 cells. Glibenclamide, an ATP-sensitive K(+) (K(ATP)(+)) channel inhibitor, did not affect the protective effect of NaHS, although mRNAs for K(ATP)(+) channel subunits, Kir6.1 and SUR1, were detected in RGM1 cells. In anesthetized rats, oral administration of NaHS protected against gastric mucosal lesion caused by ischemia-reperfusion. These results suggest that NaHS/H(2)S may protect gastric mucosal epithelial cells against oxidative stress through stimulation of MAP kinase pathways, a therapeutic dose range being very narrow. |
| 巻・号 | 241(1-2) |
| ページ | 11-8 |
| 公開日 | 2007-11-20 |
| DOI | 10.1016/j.tox.2007.07.020 |
| PII | S0300-483X(07)00488-X |
| PMID | 17825973 |
| MeSH | ATP-Binding Cassette Transporters / metabolism Air Pollutants / pharmacology* Animals Apoptosis / drug effects Bisbenzimidazole Cell Death / drug effects Cell Line Epithelial Cells / drug effects* Extracellular Signal-Regulated MAP Kinases / metabolism Gastric Mucosa / drug effects Gastric Mucosa / pathology* Glyburide / pharmacology Hydrogen Peroxide / toxicity Hydrogen Sulfide / pharmacology* Hypoglycemic Agents / pharmacology JNK Mitogen-Activated Protein Kinases / metabolism KATP Channels / metabolism Multidrug Resistance-Associated Proteins / metabolism Oxidants / toxicity Oxidative Stress / drug effects* Potassium Channels, Inwardly Rectifying / metabolism RNA, Messenger / biosynthesis RNA, Messenger / genetics Rats Receptors, Drug Reperfusion Injury / pathology Reperfusion Injury / prevention & control Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / drug effects Signal Transduction / physiology Sulfonylurea Receptors |
| IF | 4.099 |
| 引用数 | 92 |
| WOS 分野 | TOXICOLOGY PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |