論文 - 詳細
| RRC ID | 4315 |
|---|---|
| 著者 | Watanabe S, Yamamoto TG, Kitagawa R. |
| タイトル | Spindle assembly checkpoint gene mdf-1 regulates germ cell proliferation in response to nutrition signals in C. elegans. |
| ジャーナル | EMBO J |
| Abstract |
When newly hatched Caenorhabditis elegans larvae are starved, their primordial germ cells (PGCs) arrest in the post-S phase. This starvation-induced PGC arrest is mediated by the DAF-18/PTEN-AKT-1/PKB nutrient-sensing pathway. Here, we report that the conserved spindle assembly checkpoint (SAC) component MDF-1/MAD1 is required for the PGC arrest. We identified 2 Akt kinase phosphorylation sites on MDF-1. Expression of a non-phosphorylatable mutant MDF-1 partially suppressed the defect in the starvation-induced PGC arrest in L1 larvae lacking DAF-18, suggesting that MDF-1 regulates germ cell proliferation as a downstream target of AKT-1, thereby demonstrating a functional link between cell-cycle regulation by the SAC components and nutrient sensing by DAF-18-AKT-1 during post-embryonic development. The phosphorylation status of MDF-1 affects its binding to another SAC component, MDF-2/MAD2. The loss of MDF-2 or another SAC component also caused inappropriate germ cell proliferation, but the defect was less severe than that caused by mdf-1 hemizygosity, suggesting that MDF-1 causes the PGC arrest by two mechanisms, one involving MDF-2 and another that is independent of other SAC components. |
| 巻・号 | 27(7) |
| ページ | 1085-96 |
| 公開日 | 2008-4-9 |
| DOI | 10.1038/emboj.2008.32 |
| PII | emboj200832 |
| PMID | 18309291 |
| PMC | PMC2323251 |
| MeSH | Alleles Animals Apoptosis Caenorhabditis elegans / cytology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / genetics* Cell Proliferation Food* Food Deprivation Germ Cells / cytology* Germ Cells / enzymology Heterozygote Homozygote Larva Models, Biological Mutation / genetics Phosphorylation Protein Binding Proto-Oncogene Proteins c-akt / metabolism Signal Transduction* Spindle Apparatus / metabolism* Threonine / metabolism |
| IF | 9.889 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm2190 |