Reference - Detail
| RRC ID | 4319 |
|---|---|
| Author | Yamashiro S, Cox EA, Baillie DL, Hardin JD, Ono S. |
| Title | Sarcomeric actin organization is synergistically promoted by tropomodulin, ADF/cofilin, AIP1 and profilin in C. elegans. |
| Journal | J Cell Sci |
| Abstract |
Sarcomeric organization of thin and thick filaments in striated muscle is important for the efficient generation of contractile forces. Sarcomeric actin filaments are uniform in their lengths and regularly arranged in a striated pattern. Tropomodulin caps the pointed end of actin filaments and is a crucial regulator of sarcomere assembly. Here, we report unexpected synergistic functions of tropomodulin with enhancers of actin filament dynamics in Caenorhabditis elegans striated muscle. Pointed-end capping by tropomodulin inhibited actin filament depolymerization by ADF/cofilin in vitro. However, in vivo, the depletion of tropomodulin strongly enhanced the disorganization of sarcomeric actin filaments in ADF/cofilin mutants, rather than antagonistically suppressing the phenotype. Similar phenotypic enhancements by tropomodulin depletion were also observed in mutant backgrounds for AIP1 and profilin. These in vivo effects cannot be simply explained by antagonistic effects of tropomodulin and ADF/cofilin in vitro. Thus, we propose a model in which tropomodulin and enhancers of actin dynamics synergistically regulate elongation and shortening of actin filaments at the pointed end. |
| Volume | 121(Pt 23) |
| Pages | 3867-77 |
| Published | 2008-12-1 |
| DOI | 10.1242/jcs.040477 |
| PII | jcs.040477 |
| PMID | 18984629 |
| PMC | PMC2615493 |
| MeSH | Actin Cytoskeleton / metabolism* Actin Cytoskeleton / ultrastructure Actin Depolymerizing Factors / metabolism* Actins / metabolism Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Microfilament Proteins / metabolism* Microscopy, Fluorescence Models, Molecular Mutation Phenotype Profilins / metabolism* Sarcomeres / metabolism* Sarcomeres / ultrastructure Tropomodulin / genetics Tropomodulin / metabolism* |
| IF | 4.573 |
| Times Cited | 26 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Policy sources |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm724 tm1362 |