論文 - 詳細
| RRC ID | 43200 |
|---|---|
| 著者 | Hirata I, Nomura Y, Ito M, Shimazu A, Okazaki M. |
| タイトル | Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds. |
| ジャーナル | J Artif Organs |
| Abstract |
The development is expected of scaffold biomaterials that feature a shape-maintaining property in addition to high porosity and large pores that cells can easily invade. To develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized carbonate apatite (CO3Ap) was mixed with neutralized collagen gel, and the CO3Ap-collagen mixtures were lyophilized into sponges in a porous hydroxyapatite (HAp) frame ring. X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR) analyses together with chemical analysis indicated that the synthesized CO3Ap had a crystalline nature and a chemical composition similar to that of bone. Scanning electron microscope (SEM) observation showed that the CO3Ap-collagen sponge had a sui pore size for cell invasion. In proliferation and differentiation experiments with osteoblasts, alkaline phosphatase and osteopontin activity were clearly detected. When these sponge-frame complexes with bone morphogenic protein (rh-BMP2) were implanted beneath the periosteum cranii of rats, significant new bone was created at the surface of the periosteum cranii after 4 weeks of implantation. These reinforced CO3Ap-collagen sponges with rh-BMP2 are expected to be used as hard tissue scaffold biomaterials for the therapeutic purpose of the rapid cure of bone defects. |
| 巻・号 | 10(4) |
| ページ | 212-7 |
| 公開日 | 2007-1-1 |
| DOI | 10.1007/s10047-007-0391-2 |
| PMID | 18071850 |
| MeSH | Absorbable Implants* Animals Apatites / chemistry* Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / pharmacology* Carbonates / chemistry* Cattle Cell Culture Techniques Collagen / chemistry* Gels Humans Male Mice Microscopy, Electron, Scanning Osteogenesis* Porosity Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Spectroscopy, Fourier Transform Infrared Tissue Engineering* Transforming Growth Factor beta / pharmacology* X-Ray Diffraction |
| IF | 1.223 |
| 引用数 | 17 |
| WOS 分野 | TRANSPLANTATION ENGINEERING, BIOMEDICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |