RRC ID 43200
Author Hirata I, Nomura Y, Ito M, Shimazu A, Okazaki M.
Title Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds.
Journal J Artif Organs
Abstract The development is expected of scaffold biomaterials that feature a shape-maintaining property in addition to high porosity and large pores that cells can easily invade. To develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized carbonate apatite (CO3Ap) was mixed with neutralized collagen gel, and the CO3Ap-collagen mixtures were lyophilized into sponges in a porous hydroxyapatite (HAp) frame ring. X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR) analyses together with chemical analysis indicated that the synthesized CO3Ap had a crystalline nature and a chemical composition similar to that of bone. Scanning electron microscope (SEM) observation showed that the CO3Ap-collagen sponge had a sui pore size for cell invasion. In proliferation and differentiation experiments with osteoblasts, alkaline phosphatase and osteopontin activity were clearly detected. When these sponge-frame complexes with bone morphogenic protein (rh-BMP2) were implanted beneath the periosteum cranii of rats, significant new bone was created at the surface of the periosteum cranii after 4 weeks of implantation. These reinforced CO3Ap-collagen sponges with rh-BMP2 are expected to be used as hard tissue scaffold biomaterials for the therapeutic purpose of the rapid cure of bone defects.
Volume 10(4)
Pages 212-7
Published 2007-1-1
DOI 10.1007/s10047-007-0391-2
PMID 18071850
MeSH Absorbable Implants* Animals Apatites / chemistry* Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / pharmacology* Carbonates / chemistry* Cattle Cell Culture Techniques Collagen / chemistry* Gels Humans Male Mice Microscopy, Electron, Scanning Osteogenesis* Porosity Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Spectroscopy, Fourier Transform Infrared Tissue Engineering* Transforming Growth Factor beta / pharmacology* X-Ray Diffraction
IF 1.223
Times Cited 17
Human and Animal Cells MC3T3-E1(RCB1126)