RRC ID 43203
Author Kanemura N, Tsurumi H, Okuno M, Matsushima-Nishiwaki R, Shimizu M, Moriwaki H.
Title Retinoid X receptor alpha is highly phosphorylated in retinoic acid-resistant HL-60R cells and the combination of 9-cis retinoic acid plus MEK inhibitor induces apoptosis in the cells.
Journal Leuk. Res.
Abstract We examined the effects of 9-cis retinoic acid (RA) on the expression levels of retinoid X receptor alpha (RXR alpha) and its phosphorylated form (p-RXR alpha) in HL-60 and HL-60R cells. 9-cis RA reduced both RXR alpha and p-RXR alpha in HL-60 cells, but did neither in HL-60R cells. However, when the HL-60R cells were treated with the combination of 9-cis RA plus PD98059, MEK inhibitor, the p-RXR alpha and RXR alpha proteins all markedly decreased. Moreover, the combination of those agents induced apoptosis in HL-60R cells. Phosphorylation of RXR alpha might be associated with RA-resistance in HL-60R cells.
Volume 32(6)
Pages 884-92
Published 2008-6
DOI 10.1016/j.leukres.2007.11.005
PII S0145-2126(07)00438-9
PMID 18082883
MeSH Alitretinoin Apoptosis / drug effects* Apoptosis / physiology Blotting, Western Butadienes / pharmacology Cell Proliferation / drug effects Drug Resistance, Neoplasm* Drug Therapy, Combination Enzyme Inhibitors / pharmacology Flavonoids / pharmacology HL-60 Cells Humans Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors* Mitogen-Activated Protein Kinase Kinases / metabolism Nitriles / pharmacology Phosphorylation / drug effects Retinoid X Receptor alpha / metabolism* Tretinoin / pharmacology*
IF 2.066
Times Cited 9
Human and Animal Cells