| RRC ID |
43240
|
| 著者 |
Yamaguchi A, Gabazza EC, Takei Y, Yano Y, Fujimoto H, D'Alessandro-Gabazza CN, Murakami E, Kobayashi T, Takagi T, Maruyama J, Suzuki K, Taguchi O.
|
| タイトル |
Role of thrombin in interleukin-5 expression from basophils.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Interleukin-5 (IL-5) plays a key role in the pathogenesis of bronchial asthma. Thrombin is a procoagulant factor that has been also reported to participate in the inflammatory response by stimulating the secretion of cytokines. Interaction of inflammatory cells with airway epithelial cells may also promote the secretion of cytokines. However, the role of thrombin and cell-to-cell interaction in pathogenesis of allergic inflammation is unclear. In this study, we evaluated the role of thrombin and cell-to-cell interaction in the secretion of IL-5 from basophils. The human basophil cell line KU-812 was used in the assays. Thrombin and co-culture with alveolar epithelial cells significantly stimulated the secretion of IL-5 from KU-812 cells as compared to controls. Secretion of IL-5 was synergistically stimulated when KU-812 cells were incubated in the presence of both thrombin and alveolar epithelial cells. Co-culture of KU-812 cells with epithelial cells significantly increased the expression of tissue factor, an activator of coagulation activation, in a cell dose-dependent manner. Secretion of IL-5 from KU-812 basophils co-cultured with epithelial cells was significantly inhibited by LY294002, an inhibitor of phosphatidylinositol 3-kinase. These results suggest that thrombin and cell interaction with lung epithelial cells may augment the inflammatory response in allergic diseases by stimulating the secretion of IL-5 from basophils.
|
| 巻・号 |
368(1)
|
| ページ |
116-20
|
| 公開日 |
2008-3-28
|
| DOI |
10.1016/j.bbrc.2008.01.048
|
| PII |
S0006-291X(08)00087-9
|
| PMID |
18206982
|
| MeSH |
Basophils / drug effects*
Basophils / metabolism*
Cell Line
Coculture Techniques
Epithelial Cells / drug effects
Epithelial Cells / metabolism
Humans
Interleukin-5 / metabolism*
Phosphatidylinositol 3-Kinases / metabolism
Phosphoinositide-3 Kinase Inhibitors
Protein Kinase Inhibitors / pharmacology
Receptors, Thrombin / metabolism
Thrombin / pharmacology*
|
| IF |
2.985
|
| 引用数 |
4
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
KU812(RCB0495) |
