RRC ID 43423
Author Nakamura K, Miyazato A, Koguchi Y, Adachi Y, Ohno N, Saijo S, Iwakura Y, Takeda K, Akira S, Fujita J, Ishii K, Kaku M, Kawakami K.
Title Toll-like receptor 2 (TLR2) and dectin-1 contribute to the production of IL-12p40 by bone marrow-derived dendritic cells infected with Penicillium marneffei.
Journal Microbes Infect.
Abstract The present study was designed to elucidate the role of TLR2, TLR4 and dectin-1 in the production of IL-12p40 by bone marrow-derived dendritic cells (BM-DCs) infected with Penicillium marneffei. IL-12p40 production was almost completely abrogated in BM-DCs from TLR2 gene-knockout (KO) and MyD88KO mice, but not from TLR4-defective C3H/HeJ mice compared to those from control mice. Furthermore, BM-DCs from dectin-1KO mice faintly produced IL-12p40 upon stimulation with this fungus. Using a luciferase reporter assay, P. marneffei activated NF-kappaB in HEK293 cells transfected with the TLR2 gene, but not with the dectin-1 gene, and their co-transfection did not lead to further increase in this response. These results indicate that TLR2 and dectin-1 are essential in sensing P. marneffei for the activation of BM-DCs.
Volume 10(10-11)
Pages 1223-7
Published 2008-8
DOI 10.1016/j.micinf.2008.06.011
PII S1286-4579(08)00173-1
PMID 18652908
MeSH Analysis of Variance Animals Bone Marrow Cells / immunology Bone Marrow Cells / metabolism Bone Marrow Cells / microbiology Dendritic Cells / immunology* Dendritic Cells / metabolism Dendritic Cells / microbiology* Interleukin-12 Subunit p40 / biosynthesis* Lectins, C-Type Luciferases Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mice, Inbred C57BL Mice, Knockout NF-kappa B / metabolism Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Penicillium / immunology* Penicillium / metabolism Penicillium / pathogenicity Toll-Like Receptor 2 / genetics Toll-Like Receptor 2 / metabolism* Toll-Like Receptor 4 / genetics Toll-Like Receptor 4 / metabolism
IF 2.924
Times Cited 10
WOS Category INFECTIOUS DISEASES IMMUNOLOGY MICROBIOLOGY
Resource
Human and Animal Cells