Reference - Detail
|Author||Nakamura K, Miyazato A, Koguchi Y, Adachi Y, Ohno N, Saijo S, Iwakura Y, Takeda K, Akira S, Fujita J, Ishii K, Kaku M, Kawakami K.|
|Title||Toll-like receptor 2 (TLR2) and dectin-1 contribute to the production of IL-12p40 by bone marrow-derived dendritic cells infected with Penicillium marneffei.|
The present study was designed to elucidate the role of TLR2, TLR4 and dectin-1 in the production of IL-12p40 by bone marrow-derived dendritic cells (BM-DCs) infected with Penicillium marneffei. IL-12p40 production was almost completely abrogated in BM-DCs from TLR2 gene-knockout (KO) and MyD88KO mice, but not from TLR4-defective C3H/HeJ mice compared to those from control mice. Furthermore, BM-DCs from dectin-1KO mice faintly produced IL-12p40 upon stimulation with this fungus. Using a luciferase reporter assay, P. marneffei activated NF-kappaB in HEK293 cells transfected with the TLR2 gene, but not with the dectin-1 gene, and their co-transfection did not lead to further increase in this response. These results indicate that TLR2 and dectin-1 are essential in sensing P. marneffei for the activation of BM-DCs.
|MeSH||Analysis of Variance Animals Bone Marrow Cells / immunology Bone Marrow Cells / metabolism Bone Marrow Cells / microbiology Dendritic Cells / immunology* Dendritic Cells / metabolism Dendritic Cells / microbiology* Interleukin-12 Subunit p40 / biosynthesis* Lectins, C-Type Luciferases Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mice, Inbred C57BL Mice, Knockout NF-kappa B / metabolism Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Penicillium / immunology* Penicillium / metabolism Penicillium / pathogenicity Toll-Like Receptor 2 / genetics Toll-Like Receptor 2 / metabolism* Toll-Like Receptor 4 / genetics Toll-Like Receptor 4 / metabolism|
|WOS Category||INFECTIOUS DISEASES IMMUNOLOGY MICROBIOLOGY|
|Human and Animal Cells|