RRC ID 43502
Author Cheng YY, Jin H, Liu X, Siu JM, Wong YP, Ng EK, Yu J, Leung WK, Sung JJ, Chan FK.
Title Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer.
Journal Br J Cancer
Abstract Tumour suppressor genes (TSGs) were frequently inactivated through promoter hypermethylation in gastric carcinoma as well as pre-malignant gastric lesions, suggesting that promoter hypermethylation can be used as a marker to define novel TSGs and also biomarkers for early detection of gastric cancer. In an effort to search for such genes aberrantly methylated in gastric cancer development, fibulin 1 (FBLN1) was found as a candidate TSG epigenetically downregulated in gastric cancer. FBLN1 expression was downregulated in all of gastric cancer cell lines used (100%, 7 out of 7) and the primary gastric carcinoma tissues (84%, 86 out of 102) and significantly restored after pharmacological demethylation. Hypermethylation of the FBLN1 promoter was frequently (71%, 5 out of 7) detected in gastric cancer cell lines and primary gastric carcinoma tissues. Ectopic expression of FBLN1 led to the growth inhibition of gastric cancer cells through the induction of apoptosis. In summary, FBLN1 was identified as a novel candidate TSG epigenetically downregulated in gastric cancer.
Volume 99(12)
Pages 2083-7
Published 2008-12-16
DOI 10.1038/sj.bjc.6604760
PII 6604760
PMID 18985039
PMC PMC2607230
MeSH Aged Aza Compounds / pharmacology Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism* Cell Line, Tumor Cell Proliferation DNA Methylation / genetics* Down-Regulation / genetics* Female Gene Expression Regulation, Neoplastic / genetics* Humans Male Promoter Regions, Genetic / genetics* Stomach Neoplasms / genetics* Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology Up-Regulation / drug effects
IF 5.791
Times Cited 54
Human and Animal Cells Kato III(RCB2088) MKN28(RCB1000) MKN45(RCB1001)