RRC ID 43523
Author Kuwabara Y, Yamada T, Yamazaki K, Du WL, Banno K, Aoki D, Sakamoto M.
Title Establishment of an ovarian metastasis model and possible involvement of E-cadherin down-regulation in the metastasis.
Journal Cancer Sci
Abstract Clinical observations of cases of ovarian metastasis suggest that there may be a unique mechanism underlying ovarian-specific metastasis. This study was undertaken to establish an in vivo model of metastasis to the ovary, and to investigate the mechanism of ovarian-specific metastasis. We examined the capacity for ovarian metastasis in eight different human carcinoma cell lines by implantation in female NOD/SCID mice transvenously and intraperitoneally. By transvenous inoculation, only RERF-LC-AI, a poorly differentiated carcinoma cell line, frequently demonstrated ovarian metastasis. By intraperitoneal inoculation, four of the eight cell lines (HGC27, MKN-45, KATO-III, and RERF-LC-AI) metastasized to the ovary. We compared E-cadherin expression among ovarian metastatic cell lines and others. All of these four ovarian metastatic cell lines and HSKTC, a Krukenberg tumor cell line, showed E-cadherin down-regulation and others did not. E-cadherin was then forcibly expressed in RERF-LC-AI, and inhibited ovarian metastasis completely. The capacity for metastasizing to the other organs was not affected by E-cadherin expression. We also performed histological investigation of clinical ovarian-metastatic tumor cases. About half of all ovarian-metastatic tumor cases showed loss or reduction of E-cadherin expression. These data suggest that E-cadherin down-regulation may be involved in ovarian-specific metastasis.
Volume 99(10)
Pages 1933-9
Published 2008-10-1
DOI 10.1111/j.1349-7006.2008.00946.x
PMID 19016752
MeSH Animals Cadherins / genetics* Cadherins / metabolism Carcinoma / genetics Carcinoma / metabolism Carcinoma / pathology Cell Line, Tumor Disease Models, Animal* Down-Regulation* Female Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Mice Mice, Inbred NOD Mice, SCID Neoplasm Metastasis Ovarian Neoplasms / genetics Ovarian Neoplasms / metabolism* Ovarian Neoplasms / pathology* Xenograft Model Antitumor Assays
IF 4.966
Times Cited 20
Human and Animal Cells MKN28(RCB1000) MKN45(RCB1001) MKN74(RCB1002) Kato III(RCB2088) HGC-27(RCB0500) HSKTC(RCB0515) RERF-LC-AI(RCB0444)