Reference - Detail
| RRC ID | 43536 |
|---|---|
| Author | Yamasaki Y, Katsuo D, Nakayasu S, Salati C, Duan J, Zou Y, Matsuyama Y, Yamaguchi K, Oda T. |
| Title | Purification and characterization of a novel high molecular weight exotoxin produced by red tide phytoplankton, Alexandrium tamarense. |
| Journal | J Biochem Mol Toxicol |
| Abstract |
Our recent studies have demonstrated that the aqueous extract prepared from Alexandrium tamarense, a harmful red tide phytoplankton, showed cytotoxicity on Vero cells. In this study, the toxic substance was purified from the culture supernatant of A. tamarense. Based on the gel-filtration profile, the molecular mass of a purified toxin was estimated to be about 1,000 kDa. On sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis, a main band with molecular mass of 1,000 kDa was detected with periodic acid-Schiff (PAS) staining, but no protein bands were detected by Coomassie brilliant blue (CBB) protein staining. Sugar composition analysis of the toxin suggested that the toxin contains galactose, fucose, mannose, N-acetylglucosamine, xylose, and other minor saccharides, whereas no significant levels of amino acids were detected by amino acid analysis. These results suggest that the toxin is a polysaccharide-based compound. The toxin showed cytotoxic effects on various cell lines in a concentration-dependent manner. Among the cell lines tested, U937 cells were the most susceptible to the toxin. In U937 cells treated with the toxin, a typical apoptotic nuclear morphological change and DNA fragmentation were observed. This is the first report demonstrating that a polysaccharide-based toxin isolated from red tide phytoplankton can induce apoptotic cell death. |
| Volume | 22(6) |
| Pages | 405-15 |
| Published | 2008-1-1 |
| DOI | 10.1002/jbt.20253 |
| PMID | 19111002 |
| MeSH | Animals Cell Death / drug effects Cell Nucleus / drug effects Cell Nucleus / metabolism Cell-Free System Cells, Cultured Chlorocebus aethiops Chromatography, Gel Chromatography, High Pressure Liquid DNA Fragmentation / drug effects Dinoflagellida / chemistry* Dinoflagellida / cytology Enzyme Inhibitors / pharmacology Exotoxins / chemistry Exotoxins / isolation & purification* Exotoxins / metabolism* Exotoxins / pharmacology HeLa Cells Hemolysis / drug effects Humans Molecular Weight Phytoplankton / chemistry* Phytoplankton / cytology Polysaccharides / chemistry Rabbits Subcellular Fractions / drug effects Subcellular Fractions / metabolism U937 Cells Vero Cells |
| IF | 3.606 |
| Times Cited | 18 |
| WOS Category | TOXICOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | U-937 DE-4(RCB0435) MDCK(RCB0995) XC(RCB1831) Vero(RCB0001) HeLa(RCB0007) |