| RRC ID |
43548
|
| 著者 |
Noguchi C, Umino T, Miyazaki Y, Jinta T, Usui Y, Yoshizawa Y.
|
| タイトル |
TGF-beta and glutathione promote tissue repair in cigarette smoke induced injury.
|
| ジャーナル |
J Med Dent Sci
|
| Abstract |
We studied the effect of cigarette smoke extract (CSE) on a three-dimensional (3-D) co-culture model with epithelial cells and mesenchymal cells to clarify how epithelial cells protect lung tissue from cigarette smoke in-vivo. Two types of gels were prepared. The one was the co-culture of human fetal lung fibroblasts (HFL-I) embedded in type-I collagen gel, with alveolar epithelial cells (A549) cultured covering the top of the gel. The other was HFL-I cells alone. After 48 hours from CSE exposure, gel contraction, levels of fibronectin, transforming growth factor (TGF)-beta and GSH were assessed. CSE inhibited fibroblast-mediated gel contraction and this inhibition was lessened in co-culture associated with higher GSH concentration and TGF-beta1 level as compared to the level in HFL-I cells alone. CSE lowered fibronectin level to a lesser extent in co-culture as compared to the level in HFL-I cells alone. Exogenous TGF-beta1 restored the inhibition of gel contraction by CSE independent of GSH level. Cigarette smoke may interfere with 3-D co-culture gel contraction by diminishing GSH, fibronectin and TGF-beta1 action in the epithelial-mesenchymal interaction.
|
| 巻・号 |
54(1)
|
| ページ |
109-16
|
| 公開日 |
2007-3-1
|
| PMID |
19845143
|
| MeSH |
Cells, Cultured
Coculture Techniques
Epithelial Cells / metabolism
Epithelial Cells / pathology
Fibroblasts / metabolism
Fibroblasts / pathology
Fibronectins / metabolism
Glutathione / metabolism*
Humans
Lung / cytology
Lung / metabolism*
Lung / pathology
Recombinant Proteins / metabolism
Regeneration / physiology*
Smoke / adverse effects*
Tobacco
Transforming Growth Factor beta / metabolism*
|
| リソース情報 |
| ヒト・動物細胞 |
HFL-I(RCB0521) |
