論文 - 詳細
| RRC ID | 43548 |
|---|---|
| 著者 | Noguchi C, Umino T, Miyazaki Y, Jinta T, Usui Y, Yoshizawa Y. |
| タイトル | TGF-beta and glutathione promote tissue repair in cigarette smoke induced injury. |
| ジャーナル | J Med Dent Sci |
| Abstract |
We studied the effect of cigarette smoke extract (CSE) on a three-dimensional (3-D) co-culture model with epithelial cells and mesenchymal cells to clarify how epithelial cells protect lung tissue from cigarette smoke in-vivo. Two types of gels were prepared. The one was the co-culture of human fetal lung fibroblasts (HFL-I) embedded in type-I collagen gel, with alveolar epithelial cells (A549) cultured covering the top of the gel. The other was HFL-I cells alone. After 48 hours from CSE exposure, gel contraction, levels of fibronectin, transforming growth factor (TGF)-beta and GSH were assessed. CSE inhibited fibroblast-mediated gel contraction and this inhibition was lessened in co-culture associated with higher GSH concentration and TGF-beta1 level as compared to the level in HFL-I cells alone. CSE lowered fibronectin level to a lesser extent in co-culture as compared to the level in HFL-I cells alone. Exogenous TGF-beta1 restored the inhibition of gel contraction by CSE independent of GSH level. Cigarette smoke may interfere with 3-D co-culture gel contraction by diminishing GSH, fibronectin and TGF-beta1 action in the epithelial-mesenchymal interaction. |
| 巻・号 | 54(1) |
| ページ | 109-16 |
| 公開日 | 2007-3-1 |
| PMID | 19845143 |
| MeSH | Cells, Cultured Coculture Techniques Epithelial Cells / metabolism Epithelial Cells / pathology Fibroblasts / metabolism Fibroblasts / pathology Fibronectins / metabolism Glutathione / metabolism* Humans Lung / cytology Lung / metabolism* Lung / pathology Recombinant Proteins / metabolism Regeneration / physiology* Smoke / adverse effects* Tobacco Transforming Growth Factor beta / metabolism* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HFL-I(RCB0521) |