RRC ID 43586
Author Kulkeaw K, Inoue T, Mizuochi C, Horio Y, Ishihama Y, Sugiyama D.
Title Ectopic expression of Hmgn2 antagonizes mouse erythroid differentiation in vitro.
Journal Cell Biol Int
Abstract Hmgn2 (high mobility group nucleosomal 2), a ubiquitous nucleosome-binding protein that unfolds chromatin fibres and enhances DNA replication, reportedly regulates differentiation of epithelial and mesenchymal cells. To investigate how Hmgn2 regulates HC (haemopoietic cell) differentiation, we quantified Hmgn2 expression in HCs of mouse FL (fetal liver) during erythroid differentiation. Hmgn2 expression levels were >10-fold higher in immature erythroid progenitors than in mature erythroid cells, suggesting that Hmgn2 antagonizes erythroid differentiation. To address this issue, Hmgn2 were transfected into both Friend erythroleukaemia cells and FL HCs. There was a 3.3-fold decrease in relatively mature c-Kit(+)/CD71(+) erythroid cells, a 2.9-fold increase in immature c-Kit(+)/CD71(-) erythroid cells in transfected Friend cells, a 1.1-fold decrease in relatively mature CD71(+)/Ter119(+) erythroid cells, and a 1.7-fold increase in relatively immature c-Kit(+)/CD71(+) erythroid cells in FL HCs accompanied by down-regulation of genes encoding the erythroid transcription factors, Gata1 and Klf1. Two days after Hmgn2 transfection of Friend erythroleukaemia cells, the number of S-phase cells increased, whereas the number of cells in G(1) decreased, while that of mitotic cells remained unchanged. We conclude that ectopic expression of Hmgn2 antagonizes mouse erythroid differentiation in vitro, which may be due to enhancement of DNA replication and/or blocking entry of mitosis at S-phase.
Volume 36(2)
Pages 195-202
Published 2012-2-1
DOI 10.1042/CBI20110169
PII CBI20110169
PMID 21988615
MeSH Animals Antigens, CD / genetics Antigens, CD / metabolism Blood Group Antigens / genetics Blood Group Antigens / metabolism Cell Differentiation / physiology* Cell Line, Tumor Cells, Cultured Erythroid Cells / cytology* GATA1 Transcription Factor / metabolism Gene Expression Profiling HMGN2 Protein / genetics HMGN2 Protein / metabolism* Kruppel-Like Transcription Factors / metabolism Liver / metabolism Mice Mice, Inbred C57BL Mitosis Proto-Oncogene Proteins c-kit / genetics Proto-Oncogene Proteins c-kit / metabolism Receptors, Transferrin / genetics Receptors, Transferrin / metabolism S Phase
IF 2.571
Times Cited 3
Human and Animal Cells F5-5.fl(RCB0560)