| RRC ID |
43830
|
| 著者 |
Mase Y, Ishibashi O, Ishikawa T, Takizawa T, Kiguchi K, Ohba T, Katabuchi H, Takeshita T, Takizawa T.
|
| タイトル |
MiR-21 is enriched in the RNA-induced silencing complex and targets COL4A1 in human granulosa cell lines.
|
| ジャーナル |
Reprod Sci
|
| Abstract |
MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and found that miR-21 accounted for more than 80% of EIF2C2-bound miRNAs, suggesting that it was enriched in the RNA-induced silencing complex (RISC) and played a functional role in human granulosa cell (GC) lines. We also found high expression levels of miR-21 in primary human GCs. Assuming that miR-21 target mRNAs are enriched in RISC, we performed cDNA cloning of EIF2C2-bound mRNAs in KGN cells. We identified COL4A1 mRNA as a miR-21 target in the GC lines. These data suggest that miR-21 is involved in the regulation of the synthesis of COL4A1, a component of the basement membrane surrounding the GC layer and granulosa-embedded extracellular structure.
|
| 巻・号 |
19(10)
|
| ページ |
1030-40
|
| 公開日 |
2012-10-1
|
| DOI |
10.1177/1933719112442245
|
| PII |
1933719112442245
|
| PMID |
22573493
|
| MeSH |
Argonaute Proteins / genetics
Argonaute Proteins / metabolism
Carboxypeptidases / metabolism*
Cell Line
Collagen Type IV / biosynthesis*
Female
Gene Expression Profiling
Granulosa Cells / metabolism*
Humans
MicroRNAs / analysis
MicroRNAs / genetics
MicroRNAs / metabolism*
|
| IF |
2.616
|
| 引用数 |
17
|
|
WOS 分野
|
OBSTETRICS & GYNECOLOGY
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
KGN(RCB1154)
293(RCB1637) |
