論文 - 詳細
| RRC ID | 43830 |
|---|---|
| 著者 | Mase Y, Ishibashi O, Ishikawa T, Takizawa T, Kiguchi K, Ohba T, Katabuchi H, Takeshita T, Takizawa T. |
| タイトル | MiR-21 is enriched in the RNA-induced silencing complex and targets COL4A1 in human granulosa cell lines. |
| ジャーナル | Reprod Sci |
| Abstract |
MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and found that miR-21 accounted for more than 80% of EIF2C2-bound miRNAs, suggesting that it was enriched in the RNA-induced silencing complex (RISC) and played a functional role in human granulosa cell (GC) lines. We also found high expression levels of miR-21 in primary human GCs. Assuming that miR-21 target mRNAs are enriched in RISC, we performed cDNA cloning of EIF2C2-bound mRNAs in KGN cells. We identified COL4A1 mRNA as a miR-21 target in the GC lines. These data suggest that miR-21 is involved in the regulation of the synthesis of COL4A1, a component of the basement membrane surrounding the GC layer and granulosa-embedded extracellular structure. |
| 巻・号 | 19(10) |
| ページ | 1030-40 |
| 公開日 | 2012-10-1 |
| DOI | 10.1177/1933719112442245 |
| PII | 1933719112442245 |
| PMID | 22573493 |
| MeSH | Argonaute Proteins / genetics Argonaute Proteins / metabolism Carboxypeptidases / metabolism* Cell Line Collagen Type IV / biosynthesis* Female Gene Expression Profiling Granulosa Cells / metabolism* Humans MicroRNAs / analysis MicroRNAs / genetics MicroRNAs / metabolism* |
| IF | 2.616 |
| 引用数 | 17 |
| WOS 分野 | OBSTETRICS & GYNECOLOGY REPRODUCTIVE BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) 293(RCB1637) |