RRC ID 43900
著者 Hashizume O, Shimizu A, Yokota M, Sugiyama A, Nakada K, Miyoshi H, Itami M, Ohira M, Nagase H, Takenaga K, Hayashi J.
タイトル Specific mitochondrial DNA mutation in mice regulates diabetes and lymphoma development.
ジャーナル Proc Natl Acad Sci U S A
Abstract It has been hypothesized that respiration defects caused by accumulation of pathogenic mitochondrial DNA (mtDNA) mutations and the resultant overproduction of reactive oxygen species (ROS) or lactates are responsible for aging and age-associated disorders, including diabetes and tumor development. However, there is no direct evidence to prove the involvement of mtDNA mutations in these processes, because it is difficult to exclude the possible involvement of nuclear DNA mutations. Our previous studies resolved this issue by using an mtDNA exchange technology and showed that a G13997A mtDNA mutation found in mouse tumor cells induces metastasis via ROS overproduction. Here, using transmitochondrial mice (mito-mice), which we had generated previously by introducing G13997A mtDNA from mouse tumor cells into mouse embryonic stem cells, we provide convincing evidence supporting part of the abovementioned hypothesis by showing that G13997A mtDNA regulates diabetes development, lymphoma formation, and metastasis--but not aging--in this model.
巻・号 109(26)
ページ 10528-33
公開日 2012-6-26
DOI 10.1073/pnas.1202367109
PII 1202367109
PMID 22689997
PMC PMC3387115
MeSH 3T3 Cells Animals Base Sequence Cell Line, Transformed DNA Primers DNA, Mitochondrial / genetics* Diabetes Mellitus, Experimental / genetics* Lymphoma / genetics* Mice Mitochondrial Diseases / genetics* Mutation* Phenotype Polymerase Chain Reaction Reactive Oxygen Species / metabolism
IF 9.412
引用数 44
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
オルトメトリクス指標
オルトメトリクス指標項目
最多言及媒体 Patent(IFI CLAIMS)
各媒体での言及数の合計 4
過去6か月間でのオルトメトリクス指標の変動値 0.0
リソース情報
ヒト・動物細胞