論文 - 詳細
| RRC ID | 43976 |
|---|---|
| 著者 | Yang CR, Chen JD. |
| タイトル | Preparation and biological evaluation of chitosan-collagen-icariin composite scaffolds for neuronal regeneration. |
| ジャーナル | Neurol Sci |
| Abstract |
In this study the chitosan/collagen/icariin composite scaffolds for nerve regeneration were produced by blending and crosslinking chitosan with collagen and icariin. The microstructure of the composite scaffolds was observed by scanning electron microscopy. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide and attachment assays were conducted, respectively, to investigate the biocompatibility of the scaffolds. Cell cultural tests showed that the channel-structured porous scaffolds acted as a positive factor to support connective nerve cell growth. After culture, cells showed a clear flow trend to move close to the composite scaffolds in culture solution, arranging in spiral-like, and aligned parallel to the orientation of the channel structure on the surface of scaffolds. When compared to pure chitosan and chitosan/collagen scaffolds, Schwann cells and PC12 cells on the chitosan/collagen/icariin composite scaffolds exhibited the greatest proliferation and longest average neurite length. These results suggested that the chitosan/collagen/icariin composite scaffolds are potential cell carriers in nerve tissue engineering. |
| 巻・号 | 34(6) |
| ページ | 941-7 |
| 公開日 | 2013-6-1 |
| DOI | 10.1007/s10072-012-1165-z |
| PMID | 22833221 |
| MeSH | Animals Cell Differentiation / drug effects Cell Enlargement / drug effects* Cell Proliferation / drug effects Cells, Cultured Chitosan / chemistry Chitosan / pharmacology* Collagen / chemistry Collagen / pharmacology* Flavonoids / chemistry Flavonoids / pharmacology* Mice Mice, Inbred BALB C Microscopy, Electron, Scanning Neurites / drug effects Neurites / ultrastructure PC12 Cells Rats Schwann Cells / drug effects Schwann Cells / physiology Schwann Cells / ultrastructure Sciatic Nerve / cytology Time Factors Tissue Scaffolds* |
| IF | 2.415 |
| 引用数 | 14 |
| WOS 分野 | CLINICAL NEUROLOGY NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |